Dual Luciferase Reporter Assay System Promega

Lab Reagents

Human IgG antibody Laboratories manufactures the dual luciferase reporter assay system promega reagents distributed by Genprice. The Dual Luciferase Reporter Assay System Promega reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. To purchase these products, for the MSDS, Data Sheet, protocol, storage conditions/temperature or for the concentration, please contact luciferase assay. Other Dual products are available in stock. Specificity: Dual Category: Luciferase Group: Reporter Assay

Reporter Assay information

RARα Reporter Cellular Assay Pack

79322 2 vials
EUR 2390
Description: The RARα Reporter Cellular Assay Pack provides all the key reagents required to monitor the activity of retinoic acid receptor alpha (RARα). The pack contains the RARα Reporter (Luc)-HEK293 Cell Line, a luciferase reporter cell line that contains a firefly luciferase gene under the control of retinoic acid response elements stably integrated into HEK293 cells along with full length human RARα (GenBank Accession No. NM_000964). This cell line is functionally validated for the response to the stimulation of all-trans retinoic acid, and the expression of RARα is confirmed by Western blotting._x000D_Additionally, the pack includes cell culture medium (Thaw Medium 6) that has been optimized for use with HEK293 cells. Thaw Medium 6 includes 10% fetal bovine serum and 1% Pen/Strep. Finally, the pack provides the ONE-Step™ Luciferase Detection System. This reagent provides highly sensitive, stable detection of firefly (Photinus pyralis) luciferase activity. The ONE-Step luciferase reagent can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required.

RARβ Reporter Cellular Assay Pack

79323 2 vials
EUR 2050
Description: The RARβ Reporter Cellular Assay Pack provides all the key reagents required to monitor the activity of retinoic acid receptor beta (RARβ). The pack contains the RARβ Reporter (Luc)-HEK293 Cell Line, a luciferase reporter cell line that contains a firefly luciferase gene under the control of retinoic acid response elements stably integrated into HEK293 cells along with full length human RARα (GenBank Accession No. P10826-2). This cell line is functionally validated for the response to the stimulation of all-trans retinoic acid, and the expression of RARβ is confirmed by Western blotting._x000D_Additionally, the pack includes cell culture medium (Thaw Medium 6) that has been optimized for use with HEK293 cells. Thaw Medium 6 includes 10% fetal bovine serum and 1% Pen/Strep. Finally, the pack provides the ONE-Step™ Luciferase Detection System. This reagent provides highly sensitive, stable detection of firefly (Photinus pyralis) luciferase activity. The ONE-Step luciferase reagent can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required.

RARγ Reporter Cellular Assay Pack

79324 2 vials
EUR 2050
Description: The RARγ Reporter Cellular Assay Pack provides all the key reagents required to monitor the activity of retinoic acid receptor gamma (RARγ). The pack contains the RARγ Reporter (Luc)-HEK293 Cell Line, a luciferase reporter cell line that contains a firefly luciferase gene under the control of retinoic acid response elements stably integrated into HEK293 cells along with full length human RARα (GenBank Accession No. P13631-1). This cell line is functionally validated for the response to the stimulation of all-trans retinoic acid, and the expression of RARγ is confirmed by Western blotting._x000D_Additionally, the pack includes cell culture medium (Thaw Medium 6) that has been optimized for use with HEK293 cells. Thaw Medium 6 includes 10% fetal bovine serum and 1% Pen/Strep. Finally, the pack provides the ONE-Step™ Luciferase Detection System. This reagent provides highly sensitive, stable detection of firefly (Photinus pyralis) luciferase activity. The ONE-Step luciferase reagent can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required.

Lentiviral Dual Reporter: CMV-GFP-T2A-Luciferase plasmid (10 ug)

BLIV101PA-1 10 ug
EUR 963.6

Lentiviral Dual Reporter: CMV-GFP-T2A-Luciferase pre-packaged virus

BLIV101VA-1 >2 x10^6 IFUs
EUR 963.6

Lentiviral Dual Reporter: UBC-RFP-T2A-Luciferase plasmid (10 ug)

BLIV200PA-1 10 ug
EUR 963.6

Lentiviral Dual Reporter: UBC-RFP-T2A-Luciferase pre-packaged virus

BLIV200VA-1 >2 x10^6 IFUs
EUR 963.6

Minicircle Dual Reporter: CMV-RFP-T2A-Luciferase DNA (30 ug)

BLIV500MC-1 30 ug
EUR 859.2

Minicircle Dual Reporter: CMV-GFP-T2A-Luciferase DNA (30 ug)

BLIV501MC-1 30 ug
EUR 859.2

Minicircle Dual Reporter: EF1-RFP-T2A-Luciferase DNA (30 ug)

BLIV502MC-1 30 ug
EUR 859.2

Minicircle Dual Reporter: EF1-GFP-T2A-Luciferase DNA (30 ug)

BLIV503MC-1 30 ug
EUR 859.2

Minicircle Dual Reporter: UBC-RFP-T2A-Luciferase DNA (30 ug)

BLIV504MC-1 30 ug
EUR 859.2

Minicircle Dual Reporter: UBC-GFP-T2A-Luciferase DNA (30 ug)

BLIV505MC-1 30 ug
EUR 859.2

Minicircle Dual Reporter: MSCV-RFP-T2A-Luciferase DNA (30 ug)

BLIV506MC-1 30 ug
EUR 859.2

Minicircle Dual Reporter: MSCV-GFP-T2A-Luciferase DNA (30 ug)

BLIV507MC-1 30 ug
EUR 859.2

ONE-Step™ Luciferase Assay System - 500 ml

60690-5 500 ml
EUR 4100
Description: The One-Step Luciferase Assay System is designed to be used for high-throughput, sensitive quantitation of firefly luciferase activity in mammalian cell culture. The reagent consists of two components, a Luciferase Reagent Buffer (Component A) and Luciferase Reagent Substrate (Component B). Component A and Component B are combined to form a working solution that contains all the necessary components for cell lysis and luciferase quantitation. This assay system has several features:
• Sensitive - highly sensitive detection of firefly luciferase activity.
• Stable - the signal output is stable for more than two hours, providing flexibility with regard to incubation time
• Convenient - simple one-step, homogeneous protocol.
• High-throughput - one-step homogeneous protocol minimizes handling steps to support high-throughput screening applications
• Compatibility - works well with a variety of common media containing 0-10% serum and phenol red.
• Instrumentation - does not require a luminometer with injectors.

Bald Lentiviral Pseudovirion (Luc-eGFP Dual Reporter)

79988 500 µl x 2
EUR 795
Description: The bald lentiviral pseudovirion was produced without envelope glycoproteins such as VSV-G or SARS-CoV-2 spike. It contains a firefly luciferase and eGFP cassette (Luc-P2A-eGFP) as the reporters, driven by a CMV promoter. The bald lentiviral pseudovirion can serve as a negative control when studying virus entry initiated by specific interactions between virus particles and receptors._x000D_