Field Evaluation of the Interferon Gamma Assay for Diagnosis of Tuberculosis in Water Buffalo ( Bubalus bubalis) Comparing Four Interpretative Criteria

Field Evaluation of the Interferon Gamma Assay for Diagnosis of Tuberculosis in Water Buffalo ( Bubalus bubalis) Comparing Four Interpretative Criteria

Bovine tuberculosis (BTB) is a world zoonosis that affects many species of domestic and wild animals. Mycobatherium Bovis is the leading cause of Buffalo water infection (Bubalus bubalis) and cattle and is a great concern for human health and for Buffalo producers in Italy. The BTB eradication program is based on the oversight of the slaughterhouse and intradermal skin tests. Other in vivo diagnostic methods such as interferon-gamma dosage (IFN-γ) have been developed and are widely used in cattle to accelerate the elimination of BTB positive animals. This study is the first to evaluate the use and performance of IFN-γ analyzes, which is used as a BTB diagnostic auxiliary test in Buffalo water and presents the results of an assessment on the 2012 test. at 2019 during the BTB Buffalo eradication program in Italy.

The study involved 489 buffaloes with a positive result for the unique intradermal tuberculin test (SITT). IFN-γ tests and unique intradermal comparative tuberculin test have been used as confirmation tests. Then, a total of 458 buffaloes, high on officially tuberculosis (OTF) flocks, which have been confirmed without BTB for at least 6 years have been subjected to IFN-γ tests. In addition, to evaluate the IFN-γ test in an OTF herd with a paratuberculosis infection (PTB), 103 buffaloes have been subjected simultaneously to sitit and IFN-γ tests.

Our results have shown that the IFN-γ test in buffalo species could reach high sensitivity values ​​and specificity, and that the level of sensitivity and specificity could be chosen on the basis of the interpretative criterion and antigens used in use. the state of health of the flock and the epidemiological context of the territory. The IFN-γ test and the use of different interpretation criteria have been useful for implementing BTB diagnostic strategies in Buffalo herds, with the possibility of a flexible use of the test.

 

Intracerebroceric administration of interferon-alpha has induced depressive behaviors and neurotransmitter changes in rhesus monkeys

Interferon-Alpha (IFN-α) is a widely used cytokine in the treatment of brain cancers and viral infections with side effects, including depression. Monoamine neurotransmitter systems have been found in important roles in the depression induced by the peripheral IFN, but how the peripheral IFN-α accesses the central nervous system and contributes to the development of depression is poorly known. This study was intended to develop a non-human primate model using IFN-α intracerebroventricular administration (5 days / week for 6 weeks) to observe induced depressive type behaviors and explore Contributions from monoamine neurotransmitter systems in the development of depression. In monkeys receiving I.C.V.

The IFN-α administration, Anhédonie has been observed as a decrease in sucrose consumption, as well as symptoms resembling depressives, including increased dumbbell behaviors, a decrease in spontaneous and reactive locomotion in the cage. Home, as well as the reduction of the exploration and an increased immobile in the Open field. Central IFN-α-chronic infusion significantly increased the concentrations of cerebrospinal fluid (CSF) of noredrenaline (Na) and 3,4-dihydroxyphenylacetic acid (DOPAC), but not 5-hydroxyindoleacetic acid (5-HIAA) and homovanillic acid (HVA). These metabolites of monoamine CSF showed associations with specific behaviors related to depression.

In conclusion, the IFN-α central administration caused an anhedonia and the behaviors related to depression comparable to the results with the peripheral administration, and the development of depression was associated with the malfunction of monoamine neurotransmitters.

 Field Evaluation of the Interferon Gamma Assay for Diagnosis of Tuberculosis in Water Buffalo ( Bubalus bubalis) Comparing Four Interpretative Criteria
Field Evaluation of the Interferon Gamma Assay for Diagnosis of Tuberculosis in Water Buffalo ( Bubalus bubalis) Comparing Four Interpretative Criteria

 

Type I interferon acts as a major obstacle to the establishment of persistent infectious infectious disease infections (IBDV)

An infectious scholarship disease virus (IBDV), the best famous member of the Birnaviridae family is a very relevant avian pathogen, which causes acute and persistent infections in different avian hosts. Here we describe the establishment of clonal, long-term and productive IBDV infections in DF-1 chicken embryonic fibroblasts. Although the returns of the virus in persistently infected cells are extremely lower than those detected in extremely infected cells, the form of the replication of isolated viruses of infected cells persistently is greater than that of the parental virus.

The DF-1 and IBDV cell lines persistently and infected are derived from them not responding to the I interferon (IFN) key. The sequencing of high speed genome has revealed that this defect is due to mutations affecting the IFNα / β 2 receptor subunit (IFNAR2) gene resulting from the expression of IFNAR2 polypeptides hosting large C-terminal terminal deletions that abolish the signaling capacity of the IFNα / β receiver complex. The ectopic expression of a recombinant chicken gene IFNAR2 effectively saves the reactivity of IFNα. Curriculated Cellular Lines IBDV derived from infected persistent cells have a considerably improved susceptibility in order to establish new IBDV persistent infections. In addition, experiments carried out with human hela cells devoid of the IFNAR2 gene completely summarize the results obtained with DF-1 cells, having a very improved capacity to survive the acute AIBDV infection phase and to support the establishment of Persistent IBDV infections.

Total Protein - Human Tumor Cell Line: Jurkat

P1255815 1 mg
EUR 216

Total Protein - Murine Embryonic Stem Cell Line D3

CBA-305 500 ?g
EUR 414
Description:
  • Isolated from mouse ES-D3 cell line
  • Presented as 500 µg at 1 mg/mL in NP-40 Solubilization Buffer

Total Protein - Human Tumor Cell Line: K 562

P1255820 1 mg
EUR 216

Total Protein - Human Tumor Cell Line: MCF 7

P1255830 1 mg
EUR 216

Membrane Protein - Human Tumor Cell Line: Hela

P3255811 0.1 mg
EUR 311

HeLa/GFP Cell Line

AKR-213 1 vial
EUR 460

HeLa/Cas9 Cell Line

AKR-5111 1 vial
EUR 460

cDNA - Human Tumor Cell Line: Hela

C1255811 40 reactions
EUR 424

Genomic DNA - Human Tumor Cell Line: Hela

D1255811 100 ug
EUR 282

Total RNA - Human Tumor Cell Line: A431

R1255801-50 50 ug
EUR 229

Total RNA - Human Tumor Cell Line: Raji

R1255840-50 50 ug
EUR 229

Total RNA - Human Tumor Cell Line: Jurkat

R1255815-50 50 ug
EUR 229

Total RNA - Human Tumor Cell Line: K 562

R1255820-50 50 ug
EUR 229

Total RNA - Human Tumor Cell Line: MCF 7

R1255830-50 50 ug
EUR 229

Total Protein - Human Tumor Tissue: Bone

P1235023 1 mg
EUR 542

Total Protein - Human Tumor Tissue: Lung

P1235152 1 mg
EUR 357

Total Protein - Human Tumor Tissue: Nose

P1235178 1 mg
EUR 542

Total Protein - Human Tumor Tissue: Skin

P1235218 1 mg
EUR 357

Total Protein - Human Tumor Tissue: Brain

P1235035 1 mg
EUR 542

Total Protein - Human Tumor Tissue: Colon

P1235090 1 mg
EUR 357

Total Protein - Human Tumor Tissue: Liver

P1235149 1 mg
EUR 357

Total Protein - Human Tumor Tissue: Ovary

P1235183 1 mg
EUR 357

Total Protein - Human Tumor Tissue: Breast

P1235086 1 mg
EUR 357

Total Protein - Human Tumor Tissue: Kidney

P1235142 1 mg
EUR 357

Total Protein - Human Tumor Tissue: Oschea

P1235182 1 mg
EUR 542

Total Protein - Human Tumor Tissue: Rectum

P1235206 1 mg
EUR 357

Total Protein - Human Tumor Tissue: Spleen

P1235246 1 mg
EUR 357

Total Protein - Human Tumor Tissue: Testis

P1235260 1 mg
EUR 357

Total Protein - Human Tumor Tissue: Thymus

P1235264 1 mg
EUR 357

Total Protein - Human Tumor Tissue: Tonsil

P1235268 1 mg
EUR 542

Total Protein - Human Tumor Tissue: Ureter

P1235273 1 mg
EUR 542

Total Protein - Human Tumor Tissue: Uterus

P1235274 1 mg
EUR 357

Total Protein - Human Tumor Tissue: Adrenal

P1235004 1 mg
EUR 542

Total Protein - Human Tumor Tissue: Bladder

P1235010 1 mg
EUR 357

Total Protein - Human Tumor Tissue: Parotid

P1235190 1 mg
EUR 542

Total Protein - Human Tumor Tissue: Pharynx

P1235199 1 mg
EUR 357

Total Protein - Human Tumor Tissue: Stomach

P1235248 1 mg
EUR 357

Total Protein - Human Tumor Tissue: Thyroid

P1235265 1 mg
EUR 357

Total Protein - Human Tumor Tissue: Prostate

P1235201 1 mg
EUR 542

Total Protein - Human Tumor Tissue: Lymphoma

P1235161 1 mg
EUR 357

Total Protein - Human Tumor Tissue: Pancreas

P1235188 1 mg
EUR 542

Total Protein - Human Tumor Tissue: Melanoma

P1235218A 1 mg
EUR 542

Total Protein - Human Tumor Tissue: Esophagus

P1235106 1 mg
EUR 357

Total Protein - Human Tumor Tissue: Gallbladder

P1235118 1 mg
EUR 542

Total Protein - Human Tumor Tissue: Parathyroid

P1235189 1 mg
EUR 542

Total Protein - Human Tumor Tissue: Neurofibroma

P1235177A 1 mg
EUR 542

Total Protein - Human Tumor Tissue: Neurilemmoma

P1235177B 1 mg
EUR 542

Total Protein - Human Tumor Tissue: Leiomyosarcoma

P1235172 1 mg
EUR 542

Total Protein - Human Tumor Tissue: Synoviosarcoma

P1235257 1 mg
EUR 542

Total Protein - Human Tumor Tissue: Skeletal Muscle

P1235171 1 mg
EUR 357

Total Protein - Human Tumor Tissue: Small Intestine

P1235226 1 mg
EUR 542

Total Protein - Human Tumor Tissue: Lymphoma, Hodgkin's

P1235161A 1 mg
EUR 542

Human HeLa cells (Heat shocked) total protein lysate for western control for hsps

HSP704-C 100 ul
EUR 343.2

Human Total PSA (t-PSA) ELISA Kit

PRB-5049-TOTAL 96 assays
EUR 686.4

Human Total PSA (t-PSA) ELISA Kit

PRB-5049-TOTAL-5 5 x 96 assays
EUR 2739.6

Human PSA ELISA Combo Kit (Free + Total)

PRB-5049-C 96 assays
EUR 999.6

Human PSA ELISA Combo Kit (Free + Total)

PRB-5049-C-5 5 x 96 assays
EUR 4089.6

Total Protein - Human Tumor Tissue: Lymphoma, Non-Hodgkin's

P1235161B 1 mg
EUR 542

Total Protein - Human Tumor Tissue: Small Intestine: Duodenum

P1235101 1 mg
EUR 542

Human Total P53(Tumor Protein p53) ELISA Kit

EKF57732-48T 48T
EUR 396.9

Human Total P53(Tumor Protein p53) ELISA Kit

EKF57732-5x96T 5x96T
EUR 2693.25

Human Total P53(Tumor Protein p53) ELISA Kit

EKF57732-96T 96T
EUR 567

Human Total P53(Tumor Protein p53) ELISA Kit

EH3898 96T
EUR 628.92
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 46.875pg/ml

Matched Pair - Total Protein - Human Primary Tumor and Metastatic Tumor Tissue (PM): Colon

P8235090-PM 0.2 mg x2
EUR 1431

Matched Pair - Total Protein - Human Primary Tumor and Metastatic Tumor Tissue (PM): Liver

P8235149-PM 0.2 mg x2
EUR 1431

Matched Pair - Total Protein - Human Primary Tumor and Metastatic Tumor Tissue (PM): Lung

P8235152-PM 0.2 mg x2
EUR 1431

Human HeLa cells (normal/non-heat shocked) total protein lysate for western control for hsps

HSP703-C 100 ul
EUR 343.2

CytoSelect Clonogenic Tumor Cell Isolation Kit (5 preps)

CBA-155 5 preps
EUR 957.6
Description: Many solid tumors contain heterogeneous populations of normal and cancerous cells. Separation of these cell populations is key to an accurate assessment of the true genotypic and phenotypic differences between normal and tumor cells. Our CytoSelect Clonogenic Tumor Cell Isolation Kit uses a proprietary semisolid agar medium to facilitate formation of colonies by cells from solid tumors. Colonies are grown in either a 6-well plate or a 35mm culture dish. These colonies are isolated away from single (i.e. normal) cells by size filtration. The viable cells from these colonies can be easily recovered for further analysis.

Matched Pair - Total Protein - Human Primary Tumor and Metastatic Tumor Tissue (PM): Breast

P8235086-PM 0.2 mg x2
EUR 1431

Matched Pair - Total Protein - Human Primary Tumor and Metastatic Tumor Tissue (PM): Kidney

P8235142-PM 0.2 mg x2
EUR 1431

Matched Pair - Total Protein - Human Primary Tumor and Metastatic Tumor Tissue (PM): Rectum

P8235206-PM 0.2 mg x2
EUR 1431

Matched Pair - Total Protein - Human Primary Tumor and Metastatic Tumor Tissue (PM): Stomach

P8235248-PM 0.2 mg x2
EUR 1431

Total Cholesterol Assay Kit

STA-390 192 assays
EUR 445

Total Carbohydrate Assay Kit

STA-682 100 assays
EUR 410

Total Bile Acid Assay Kit

STA-631 100 assays
EUR 410

CytoSelect Clonogenic Tumor Cell Isolation Kit (5 x 5 preps)

CBA-155-5 25 preps
EUR 3894
Description: Many solid tumors contain heterogeneous populations of normal and cancerous cells. Separation of these cell populations is key to an accurate assessment of the true genotypic and phenotypic differences between normal and tumor cells. Our CytoSelect Clonogenic Tumor Cell Isolation Kit uses a proprietary semisolid agar medium to facilitate formation of colonies by cells from solid tumors. Colonies are grown in either a 6-well plate or a 35mm culture dish. These colonies are isolated away from single (i.e. normal) cells by size filtration. The viable cells from these colonies can be easily recovered for further analysis.

Total Thiol Assay Kit (Colorimetric)

MET-5053 192 assays
EUR 335

Membrane Protein - Human Tumor Cell Line: A431

P3255801 0.1 mg
EUR 311

Membrane Protein - Human Tumor Cell Line: Raji

P3255840 0.1 mg
EUR 311

Total Cholesterol Assay Kit (Colorimetric)

STA-384 192 assays
EUR 440

Membrane Protein - Human Tumor Cell Line: K 562

P3255820 0.1 mg
EUR 311

Membrane Protein - Human Tumor Cell Line: Jurkat

P3255815 0.1 mg
EUR 311

Fluorescent Total Cholesterol Detection Kit

FLCHOL100-2 100 Tests
EUR 345

Total Bile Acid Assay Kit (Fluorometric)

MET-5005 100 assays
EUR 410

Matched Pair - Total Protein - Human Primary Tumor and Normal Tissue (PP): Lung

P8235152-PP 0.2 mg x2
EUR 716

Matched Pair - Total Protein - Human Primary Tumor and Normal Tissue (PP): Colon

P8235090-PP 0.2 mg x2
EUR 716

Matched Pair - Total Protein - Human Primary Tumor and Normal Tissue (PP): Liver

P8235149-PP 0.2 mg x2
EUR 716

Total Phosphatidic Acid Assay Kit (Fluorometric)

MET-5019 100 assays
EUR 430

Membrane Protein - Human Tumor Cell Line: MCF 7

P3255830 0.1 mg
EUR 311

Matched Pair - Total Protein - Human Primary Tumor and Normal Tissue (PP): Breast

P8235086-PP 0.2 mg x2
EUR 716

Matched Pair - Total Protein - Human Primary Tumor and Normal Tissue (PP): Kidney

P8235142-PP 0.2 mg x2
EUR 716

Matched Pair - Total Protein - Human Primary Tumor and Normal Tissue (PP): Rectum

P8235206-PP 0.2 mg x2
EUR 716

Matched Pair - Total Protein - Human Primary Tumor and Normal Tissue (PP): Stomach

P8235248-PP 0.2 mg x2
EUR 716

OxiSelect™ Total Glutathione (GSSG/GSH) Assay Kit

STA-312 100 assays
EUR 450

Hela Cell Line

CL-0101 1×10⁶ cells/vial
EUR 420
Description: Homo sapiens, Human

OxiSelect™ Total Antioxidant Capacity (TAC) Assay Kit

STA-360 200 assays
EUR 450

FFPE Total RNA - Human Tumor: Breast

R2234086 1 ug
EUR 863

Total RNA - Human Tumor Tissue: Bone

R1235023-10 10 ug
EUR 480

Total RNA - Human Tumor Tissue: Lung

R1235152-50 50 ug
EUR 480

Total RNA - Human Tumor Tissue: Nose

R1235178-10 10 ug
EUR 480

Hela 229 cell line

CL-0634 1×10⁶ cells/vial
EUR 500
Description: Homo sapiens, human

Human HRE Reporter Cell Line–HeLa

ABC-RC0095 1 vial Ask for price
Description: Human HRE Reporter Cell Line is derived from HeLa cell. Gentaur can provide high quality reporter cell line for monitoring biological activity

Total RNA - Human Tumor Tissue: Colon

R1235090-50 50 ug
EUR 480

Total RNA - Human Tumor Tissue: Liver

R1235149-50 50 ug
EUR 480

Total RNA - Human Tumor Tissue: Ovary

R1235183-10 10 ug
EUR 480

Human ATF6 Reporter Cell Line–HeLa

ABC-RC0080 1 vial Ask for price
Description: Human ATF6 Reporter Cell Line is derived from HeLa cells. It can be used for monitoring biological activity

Total RNA - Human Tumor Tissue: Breast

R1235086-50 50 ug
EUR 480

Total RNA - Human Tumor Tissue: Kidney

R1235142-50 50 ug
EUR 480

Total RNA - Human Tumor Tissue: Rectum

R1235206-50 50 ug
EUR 480

Total RNA - Human Tumor Tissue: Thymus

R1235264-10 10 ug
EUR 480

Total RNA - Human Tumor Tissue: Tonsil

R1235268-10 10 ug
EUR 480

Total RNA - Human Tumor Tissue: Uterus

R1235274-50 50 ug
EUR 480

OxiSelect Total Glutathione (GSSG/GSH) Assay Kit, Trial Size

STA-312-T 20 assays
EUR 338.4
Description: The OxiSelect Total Glutathione Assay Kit is a quantitative assay for measuring the total glutathione content within a sample (GSH/GSSG).  Glutathione Reductase reduces oxidized glutathione (GSSG) to reduced glutathione (GSH) in the presence of NADPH.  Subsequently, the  chromogen reacts with the thiol group of GSH to produce a colored compound that absorbs at 405 nm.  The total glutathione content in unknown samples is determined by comparison with the predetermined glutathione standard curve.  The rate of chromophore production is proportional to the concentration of glutathione within the sample.  The rate can be determined from the absorbance change over time.  Metaphosphoric acid is provided to remove interfering proteins or enzymes from samples.

Total RNA - Human Tumor Tissue: Adrenal

R1235004-10 10 ug
EUR 480

Total RNA - Human Tumor Tissue: Bladder

R1235010-10 10 ug
EUR 480

Total RNA - Human Tumor Tissue: Parotid

R1235190-10 10 ug
EUR 480

Total RNA - Human Tumor Tissue: Stomach

R1235248-50 50 ug
EUR 480

Total RNA - Human Tumor Tissue: Thyroid

R1235265-50 50 ug
EUR 480

Total RNA - Human Tumor Tissue: Melanoma

R1235218A-10 10 ug
EUR 480

OxiSelect Total Antioxidant Capacity (TAC) Assay Kit, Trial Size

STA-360-T 20 assays
EUR 338.4
Description: The OxiSelect Total Antioxidant Capacity (TAC) Assay measures the total antioxidant capacity of biomolecules from a variety of samples via a SET mechanism. In the presence of antioxidants, copper(II) is reduced to copper(I). In turn, the copper(I) ions react with a chromogen to produce a color with maximum absorbance at 490nm.

Total RNA - Human Tumor Tissue: Esophagus

R1235106-50 50 ug
EUR 480

293AD Cell Line

AD-100 1 vial
EUR 365

293AAV Cell Line

AAV-100 1 vial
EUR 405

293RTV Cell Line

RV-100 1 vial
EUR 405

293LTV Cell Line

LTV-100 1 vial
EUR 405

HeLa Cell Lysate (Human epithelial Cell Line)

LF-R0001 200 ul
EUR 103.2
Description: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

Total RNA - Human Tumor Tissue: Synoviosarcoma

R1235257-10 10 ug
EUR 480

Hela S3 cell line

CL-0350 1×10⁶ cells/vial
EUR 500
Description: Homo sapiens, human

Purified Exosomes: HeLa Human Cell Line

EXOP-405A-1 50 µg
EUR 409

293/GFP Cell Line

AKR-200 1 vial
EUR 460

Total RNA - Human Tumor Tissue: Colon, sigmoid

R1235095-50 50 ug
EUR 480

T47D/GFP Cell Line

AKR-208 1 vial
EUR 686.4
Description: T47D/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

A549/GFP Cell Line

AKR-209 1 vial
EUR 460

293/Cas9 Cell Line

AKR-5110 1 vial
EUR 460

NIH3T3/GFP Cell Line

AKR-214 1 vial
EUR 460

NIH3T3/Cas9 Cell Line

AKR-5104 1 vial
EUR 460

Total RNA - Human Tumor Tissue: Small Intestine

R1235226-10 10 ug
EUR 480

Human Total tumor necrosis factor ELISA kit

E01T0034-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Human Total tumor necrosis factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Total tumor necrosis factor ELISA kit

E01T0034-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Human Total tumor necrosis factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Total tumor necrosis factor ELISA kit

E01T0034-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Human Total tumor necrosis factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Total tumor necrosis factor ELISA kit

E01A8126 96T
EUR 700
Description: ELISA

Human p53 Luciferase Reporter Cell Line- HeLa

ABC-RC0037 1 vial Ask for price
Description: Human p53 Luciferase Reporter Cell Line- HeLa is derived from human cervical cancer, and stably express firefly luciferase reporter gene under the control of the p53 response element. This cell line is an ideal cellular model for monitoring the activation of p53 Receptor Signaling Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.

Human NFAT Luciferase Reporter Cell Line- HeLa

ABC-RC0023 1 vial Ask for price
Description: Human NFAT Luciferase Reporter Cell Line- HeLa is derived from human cervical cancer, and stably express firefly luciferase reporter gene under the control of NFAT response element. This cell line is an ideal cellular model for monitoring the activation of Calcium Signaling Receptor Signaling Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.

The results presented here show here that the inactivation of the Jak-Stat signaling route significantly reduces the apoptotic response induced by infection, thus facilitating the establishment and maintenance of persistent IBDV infections. Members of the Birnaviridae family, including an infectious scholarship disease virus (IBDV). , has a double behavior, causing acute infections that are often followed by the establishment of long-service life asymptomatic infections. Indeed, persistently infected specimens could act as efficient virus tanks, so potentially contributing to the diffusion of viruses. Despite the key importance of this biological trait, information on mechanisms triggering IBDV persistence is negligible.

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