Field Evaluation of the Interferon Gamma Assay for Diagnosis of Tuberculosis in Water Buffalo ( Bubalus bubalis) Comparing Four Interpretative Criteria

Field Evaluation of the Interferon Gamma Assay for Diagnosis of Tuberculosis in Water Buffalo ( Bubalus bubalis) Comparing Four Interpretative Criteria

Bovine tuberculosis (BTB) is a world zoonosis that affects many species of domestic and wild animals. Mycobatherium Bovis is the leading cause of Buffalo water infection (Bubalus bubalis) and cattle and is a great concern for human health and for Buffalo producers in Italy. The BTB eradication program is based on the oversight of the slaughterhouse and intradermal skin tests. Other in vivo diagnostic methods such as interferon-gamma dosage (IFN-γ) have been developed and are widely used in cattle to accelerate the elimination of BTB positive animals. This study is the first to evaluate the use and performance of IFN-γ analyzes, which is used as a BTB diagnostic auxiliary test in Buffalo water and presents the results of an assessment on the 2012 test. at 2019 during the BTB Buffalo eradication program in Italy.

The study involved 489 buffaloes with a positive result for the unique intradermal tuberculin test (SITT). IFN-γ tests and unique intradermal comparative tuberculin test have been used as confirmation tests. Then, a total of 458 buffaloes, high on officially tuberculosis (OTF) flocks, which have been confirmed without BTB for at least 6 years have been subjected to IFN-γ tests. In addition, to evaluate the IFN-γ test in an OTF herd with a paratuberculosis infection (PTB), 103 buffaloes have been subjected simultaneously to sitit and IFN-γ tests.

Our results have shown that the IFN-γ test in buffalo species could reach high sensitivity values ​​and specificity, and that the level of sensitivity and specificity could be chosen on the basis of the interpretative criterion and antigens used in use. the state of health of the flock and the epidemiological context of the territory. The IFN-γ test and the use of different interpretation criteria have been useful for implementing BTB diagnostic strategies in Buffalo herds, with the possibility of a flexible use of the test.

 

Intracerebroceric administration of interferon-alpha has induced depressive behaviors and neurotransmitter changes in rhesus monkeys

Interferon-Alpha (IFN-α) is a widely used cytokine in the treatment of brain cancers and viral infections with side effects, including depression. Monoamine neurotransmitter systems have been found in important roles in the depression induced by the peripheral IFN, but how the peripheral IFN-α accesses the central nervous system and contributes to the development of depression is poorly known. This study was intended to develop a non-human primate model using IFN-α intracerebroventricular administration (5 days / week for 6 weeks) to observe induced depressive type behaviors and explore Contributions from monoamine neurotransmitter systems in the development of depression. In monkeys receiving I.C.V.

The IFN-α administration, Anhédonie has been observed as a decrease in sucrose consumption, as well as symptoms resembling depressives, including increased dumbbell behaviors, a decrease in spontaneous and reactive locomotion in the cage. Home, as well as the reduction of the exploration and an increased immobile in the Open field. Central IFN-α-chronic infusion significantly increased the concentrations of cerebrospinal fluid (CSF) of noredrenaline (Na) and 3,4-dihydroxyphenylacetic acid (DOPAC), but not 5-hydroxyindoleacetic acid (5-HIAA) and homovanillic acid (HVA). These metabolites of monoamine CSF showed associations with specific behaviors related to depression.

In conclusion, the IFN-α central administration caused an anhedonia and the behaviors related to depression comparable to the results with the peripheral administration, and the development of depression was associated with the malfunction of monoamine neurotransmitters.

 Field Evaluation of the Interferon Gamma Assay for Diagnosis of Tuberculosis in Water Buffalo ( Bubalus bubalis) Comparing Four Interpretative Criteria
Field Evaluation of the Interferon Gamma Assay for Diagnosis of Tuberculosis in Water Buffalo ( Bubalus bubalis) Comparing Four Interpretative Criteria

 

Type I interferon acts as a major obstacle to the establishment of persistent infectious infectious disease infections (IBDV)

An infectious scholarship disease virus (IBDV), the best famous member of the Birnaviridae family is a very relevant avian pathogen, which causes acute and persistent infections in different avian hosts. Here we describe the establishment of clonal, long-term and productive IBDV infections in DF-1 chicken embryonic fibroblasts. Although the returns of the virus in persistently infected cells are extremely lower than those detected in extremely infected cells, the form of the replication of isolated viruses of infected cells persistently is greater than that of the parental virus.

The DF-1 and IBDV cell lines persistently and infected are derived from them not responding to the I interferon (IFN) key. The sequencing of high speed genome has revealed that this defect is due to mutations affecting the IFNα / β 2 receptor subunit (IFNAR2) gene resulting from the expression of IFNAR2 polypeptides hosting large C-terminal terminal deletions that abolish the signaling capacity of the IFNα / β receiver complex. The ectopic expression of a recombinant chicken gene IFNAR2 effectively saves the reactivity of IFNα. Curriculated Cellular Lines IBDV derived from infected persistent cells have a considerably improved susceptibility in order to establish new IBDV persistent infections. In addition, experiments carried out with human hela cells devoid of the IFNAR2 gene completely summarize the results obtained with DF-1 cells, having a very improved capacity to survive the acute AIBDV infection phase and to support the establishment of Persistent IBDV infections.

HeLa/GFP Cell Line

AKR-213 1 vial
EUR 572
Description: HeLa/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

HeLa/Cas9 Cell Line

AKR-5111 1 vial
EUR 572

HeLa Whole Cell Lysate (Epitheloid carinoma cells)

HELA-100 100 ug
EUR 164

HeLa Whole Cell Lysate (Epitheloid carinoma cells)

HELA-50 50 ug
EUR 128

Human Total PSA (t-PSA) ELISA Kit

PRB-5049-TOTAL 96 assays
EUR 572

Human Total PSA (t-PSA) ELISA Kit

PRB-5049-TOTAL-5 5 x 96 assays
EUR 2283

Membrane Protein from Human Tumor Cell Line: Hela

P3255811 0.1 mg
EUR 285
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

cDNA from Human Tumor Cell Line: Hela

C1255811 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Human Tumor Cell Line: Hela

D1255811 100 ug
EUR 243
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total Protein - Murine Embryonic Stem Cell Line D3

CBA-305 500 ?g
EUR 345
Description:
  • Isolated from mouse ES-D3 cell line
  • Presented as 500 µg at 1 mg/mL in NP-40 Solubilization Buffer

Total Protein from Human Tumor Cell Line: A431

P1255801 1 mg
EUR 214
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Tumor Cell Line: Jurkat

P1255815 1 mg
EUR 214
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Tumor Cell Line: Raji

P1255840 1 mg
EUR 214
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Tumor Cell Line: K 562

P1255820 1 mg
EUR 214
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Tumor Cell Line: MCF 7

P1255830 1 mg
EUR 214
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total RNA from Human Tumor Cell Line: A431

R1255801-50 50 ug
EUR 194
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Cell Line: Jurkat

R1255815-50 50 ug
EUR 194
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Cell Line: Raji

R1255840-50 50 ug
EUR 194
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

HeLa Cell Lysate (Human epithelial Cell Line)

LF-R0001 200 ul
EUR 86
Description: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

Total RNA from Human Tumor Cell Line: K 562

R1255820-50 50 ug
EUR 194
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Cell Line: MCF 7

R1255830-50 50 ug
EUR 194
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

HeLa Cell Extract

30R-AG012 100 ug
EUR 349
Description: Glutathiolated human epithelial carcinoma cell (HeLa) extract

Inducible GFP Hela stable cell line

SC036 2 x 106 cell/ml x 1ml
EUR 1500
Description: InducibleGFP expression stable cell line in Hela cells, with Neomycin and puromycin resitance

Inducible RFP Hela stable cell line

SC037 2 x 106 cell/ml x 1ml
EUR 1500
Description: Inducible RFP expression stable cell line in Hela with Blasticidin and Puromycin resistance

Hela / Cas9 (Bad) Stable Cell Line

SC045-Cas9-Bsd 2 x 106 cell/ml x 1ml
EUR 1058
Description: Cas9 expression stable cell line with Blasticidin resistance in Hela human cervical cancer cells.

H2O2 stimulated HeLa Cell Lysate (Human epithelial cell line)

LF-R0002 Each 100 ul
EUR 121
Description: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate - H2O2 stimulated

Human Hela Whole Cell Lysate

LYSATE0023 200ug
EUR 150
Description: This cell lysate is prepared from human hela using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

HeLa Cell Lysate (Whole Cell)

LYSATE0001 200ug
EUR 150
Description: This whole cell lysate is prepared from Hela cells using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Total Protein from Human Tumor Tissue: Adrenal

P1235004 1 mg
EUR 461
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Tumor Tissue: Bladder

P1235010 1 mg
EUR 320
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Tumor Tissue: Bone

P1235023 1 mg
EUR 461
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Tumor Tissue: Brain

P1235035 1 mg
EUR 461
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Tumor Tissue: Breast

P1235086 1 mg
EUR 320
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Tumor Tissue: Colon

P1235090 1 mg
EUR 320
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Tumor Tissue: Esophagus

P1235106 1 mg
EUR 320
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Tumor Tissue: Gallbladder

P1235118 1 mg
EUR 461
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Tumor Tissue: Kidney

P1235142 1 mg
EUR 320
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Tumor Tissue: Liver

P1235149 1 mg
EUR 320
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Tumor Tissue: Lung

P1235152 1 mg
EUR 320
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Tumor Tissue: Lymphoma

P1235161 1 mg
EUR 320
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Tumor Tissue: Leiomyosarcoma

P1235172 1 mg
EUR 461
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Tumor Tissue: Neurofibroma

P1235177A 1 mg
EUR 461
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Tumor Tissue: Neurilemmoma

P1235177B 1 mg
EUR 461
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Tumor Tissue: Nose

P1235178 1 mg
EUR 461
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Tumor Tissue: Oschea

P1235182 1 mg
EUR 461
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Tumor Tissue: Ovary

P1235183 1 mg
EUR 320
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Tumor Tissue: Pancreas

P1235188 1 mg
EUR 461
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Tumor Tissue: Parathyroid

P1235189 1 mg
EUR 461
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Tumor Tissue: Parotid

P1235190 1 mg
EUR 461
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Tumor Tissue: Pharynx

P1235199 1 mg
EUR 320
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Tumor Tissue: Prostate

P1235201 1 mg
EUR 461
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Tumor Tissue: Rectum

P1235206 1 mg
EUR 320
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Tumor Tissue: Skin

P1235218 1 mg
EUR 320
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Tumor Tissue: Melanoma

P1235218A 1 mg
EUR 461
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Tumor Tissue: Spleen

P1235246 1 mg
EUR 320
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Tumor Tissue: Stomach

P1235248 1 mg
EUR 320
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Tumor Tissue: Synoviosarcoma

P1235257 1 mg
EUR 461
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Tumor Tissue: Testis

P1235260 1 mg
EUR 320
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Tumor Tissue: Thymus

P1235264 1 mg
EUR 320
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Tumor Tissue: Thyroid

P1235265 1 mg
EUR 320
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Tumor Tissue: Tonsil

P1235268 1 mg
EUR 461
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Tumor Tissue: Ureter

P1235273 1 mg
EUR 461
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Tumor Tissue: Uterus

P1235274 1 mg
EUR 320
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Cholesterol Assay Kit

STA-390 192 assays
EUR 508
Description: Cell Biolabs? Total Cholesterol Assay Kits measure the total cholesterol within serum, plasma, cell lysate, or tissue samples. The assays will detect total cholesterol (cholesteryl esters plus free cholesterol) in the presence of cholesterol esterase or only free cholesterol in the absence of the esterase enzyme.

Total Carbohydrate Assay Kit

STA-682 100 assays
EUR 479
Description: The Total Carbohydrate Assay detects total carbohydrates within samples based on the phenol-sulfuric acid method. Total carbohydrate levels in an unknown sample are calculated based on a glucose standard curve. 100 assays/kit.

Human ; HeLa (#1)

02-723 100ng
EUR 401
Description: The Human ; HeLa (#1) is available in Europe and for worldwide shipping via Gentaur.

Human PSA ELISA Combo Kit (Free + Total)

PRB-5049-C 96 assays
EUR 833

Human PSA ELISA Combo Kit (Free + Total)

PRB-5049-C-5 5 x 96 assays
EUR 3408

Human HeLa cells (Heat shocked) total protein lysate for western control for hsps

HSP704-C 100 ul
EUR 286

Total Thiol Assay Kit (Colorimetric)

MET-5053 192 assays
EUR 409

Total Cholesterol Assay Kit (Colorimetric)

STA-384 192 assays
EUR 508
Description: Cell Biolabs? Total Cholesterol Assay Kits measure the total cholesterol within serum, plasma, cell lysate, or tissue samples. The assays will detect total cholesterol (cholesteryl esters plus free cholesterol) in the presence of cholesterol esterase or only free cholesterol in the absence of the esterase enzyme.

Total Bile Acid Assay Kit

STA-631 100 assays
EUR 479
Description: While bile acid synthesis is critical for the removal of cholesterol from the body, bile acids are also required for proper uptake of nutrients in the small intestine. The Total Bile Acid Assay Kit provides a convenient 96-well plate-based method to measure the total bile acid content in a variety of sample types.

Human Total P53(Tumor Protein p53) ELISA Kit

EH3898 96T
EUR 524.1
  • Detection range: 78.125-5000 pg/ml
  • Uniprot ID: P04637
  • Alias: TP53/BCC7/LFS1/TRP53/Antigen NY-CO-13/FLJ92943/LFS1TRP53/p53/p53 tumor suppressor/P53cellular tumor antigen p53/Phosphoprotein p53/transformation-related protein 53/tumor protein p53/Tumor
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 46.875pg/ml

Total Protein from Human Tumor Tissue: Lymphoma, Hodgkin's

P1235161A 1 mg
EUR 461
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Tumor Tissue: Skeletal Muscle

P1235171 1 mg
EUR 320
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Tumor Tissue: Small Intestine

P1235226 1 mg
EUR 461
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

HeLa cells

C0008001 One Frozen vial
EUR 455

CytoSelect Clonogenic Tumor Cell Isolation Kit (5 preps)

CBA-155 5 preps
EUR 798
Description: Many solid tumors contain heterogeneous populations of normal and cancerous cells. Separation of these cell populations is key to an accurate assessment of the true genotypic and phenotypic differences between normal and tumor cells. Our CytoSelect Clonogenic Tumor Cell Isolation Kit uses a proprietary semisolid agar medium to facilitate formation of colonies by cells from solid tumors. Colonies are grown in either a 6-well plate or a 35mm culture dish. These colonies are isolated away from single (i.e. normal) cells by size filtration. The viable cells from these colonies can be easily recovered for further analysis.

Human HeLa Whole Cell Lysate, TNFa Stimulated

HCL-2000 100ug
EUR 213

Human HeLa (Cervix Adenocarcinoma) Cell Nuclear Extract

HCL-2008 100ug
EUR 225

Human HeLa (Cervix Adenocarcinoma) Whole Cell Lysate

HCL-2009 1 mg
EUR 524

Total Bile Acid Assay Kit (Fluorometric)

MET-5005 100 assays
EUR 473
Description: Total Bile Acid Assay Kit is a colorimetric 96-well plate-based method to measure total bile acid content in a variety of sample types. The assay uses an enzyme driven reaction in which bile acids are incubated in the presence of thio-NAD+. 100 assays.

Total Phosphatidic Acid Assay Kit (Fluorometric)

MET-5019 100 assays
EUR 502
Description: Our Total Phosphatidic Acid Assay Kit measures total phosphatidic acid content (PA and LPA) in cell lysate samples by a coupled enzymatic reaction system. First, lipase is used to hydrolyze phosphatidic acid to glycerol-3-phosphate. Next, the glycerol-3-phosphate product is oxidized by glycerol-3-phosphate oxidase (GPO), producing hydrogen peroxide. The hydrogen peroxide released from this reaction reacts specifically with the kit?s Fluorometric Probe and is detected at ex. 530-560 nm/em. 585-595 nm. Phosphatidic Acid levels in unknown sampled are determined based on the provided phosphatidic acid standard curve.

Human Total tumor necrosis factor ELISA kit

E01T0034-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Human Total tumor necrosis factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Total tumor necrosis factor ELISA kit

E01T0034-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Human Total tumor necrosis factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Total tumor necrosis factor ELISA kit

E01T0034-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Human Total tumor necrosis factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Total RNA from Human Tumor Tissue: Adrenal

R1235004-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Bladder

R1235010-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Bone

R1235023-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Breast

R1235086-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Colon

R1235090-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Esophagus

R1235106-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Kidney

R1235142-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Liver

R1235149-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Lung

R1235152-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Nose

R1235178-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Ovary

R1235183-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Parotid

R1235190-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Rectum

R1235206-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Melanoma

R1235218A-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Stomach

R1235248-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Synoviosarcoma

R1235257-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Thymus

R1235264-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Thyroid

R1235265-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Tonsil

R1235268-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Uterus

R1235274-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

293AD Cell Line

AD-100 1 vial
EUR 461
Description: The 293AD Cell Line is derived from the parental 293 cells but selected for attributes that increase adenovirus production, including firmer attachment and larger surface area.

293AAV Cell Line

AAV-100 1 vial
EUR 508
Description: The 293AAV Cell Line is derived from the parental 293 cells but selected for attributes that increase AAV production, including firmer attachment and larger surface area.

293LTV Cell Line

LTV-100 1 vial
EUR 508
Description: The 293LTV Cell Line is derived from the parental 293 cells but selected for attributes that increase lentiviral production, including fimrer attachment and larger surface area.

293RTV Cell Line

RV-100 1 vial
EUR 508
Description: The 293RTV Cell Line is derived from the parental 293 cells but selected for attributes that increase retroviral production, including fimrer attachment and larger surface area.

Total Protein from Human Tumor Tissue: Small Intestine: Duodenum

P1235101 1 mg
EUR 461
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Tumor Tissue: Lymphoma, Non-Hodgkin's

P1235161B 1 mg
EUR 461
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

OxiSelect Total Glutathione (GSSG/GSH) Assay Kit

STA-312 100 assays
EUR 519
Description: The OxiSelect Total Glutathione Assay Kit is a quantitative assay for measuring the total glutathione content within a sample (GSH/GSSG).  Glutathione Reductase reduces oxidized glutathione (GSSG) to reduced glutathione (GSH) in the presence of NADPH.  Subsequently, the  chromogen reacts with the thiol group of GSH to produce a colored compound that absorbs at 405 nm.  The total glutathione content in unknown samples is determined by comparison with the predetermined glutathione standard curve.  The rate of chromophore production is proportional to the concentration of glutathione within the sample.  The rate can be determined from the absorbance change over time.  Metaphosphoric acid is provided to remove interfering proteins or enzymes from samples.

OxiSelect Total Antioxidant Capacity (TAC) Assay Kit

STA-360 200 assays
EUR 519
Description: The OxiSelect Total Antioxidant Capacity (TAC) Assay measures the total antioxidant capacity of biomolecules from a variety of samples via a SET mechanism. In the presence of antioxidants, copper(II) is reduced to copper(I). In turn, the copper(I) ions react with a chromogen to produce a color with maximum absorbance at 490nm.

Human HeLa cells (normal/non-heat shocked) total protein lysate for western control for hsps

HSP703-C 100 ul
EUR 286

CytoSelect Clonogenic Tumor Cell Isolation Kit (5 x 5 preps)

CBA-155-5 25 preps
EUR 3245
Description: Many solid tumors contain heterogeneous populations of normal and cancerous cells. Separation of these cell populations is key to an accurate assessment of the true genotypic and phenotypic differences between normal and tumor cells. Our CytoSelect Clonogenic Tumor Cell Isolation Kit uses a proprietary semisolid agar medium to facilitate formation of colonies by cells from solid tumors. Colonies are grown in either a 6-well plate or a 35mm culture dish. These colonies are isolated away from single (i.e. normal) cells by size filtration. The viable cells from these colonies can be easily recovered for further analysis.

293/GFP Cell Line

AKR-200 1 vial
EUR 572
Description: 293/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

T47D/GFP Cell Line

AKR-208 1 vial
EUR 572
Description: T47D/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

A549/GFP Cell Line

AKR-209 1 vial
EUR 572
Description: A549/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

NIH3T3/GFP Cell Line

AKR-214 1 vial
EUR 572
Description: NIH3T3/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

NIH3T3/Cas9 Cell Line

AKR-5104 1 vial
EUR 572

293/Cas9 Cell Line

AKR-5110 1 vial
EUR 572

Total RNA from Human Normal Lymphocyte Cell Line

R1254148-1 100 ug
EUR 228
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Normal Lymphocyte Cell Line

R1254148-2 4 x 100 ug
EUR 644
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

HeLa Nuclear Extract

1641-01
EUR 185

HeLa Nuclear Extract

1641-1
EUR 338

Chang Liver (HeLa)

C0050007 One Frozen vial
EUR 543

HeLa Antigen Concentrate

F813X 1 ml
EUR 527
  • Product category: Quality control/standard for molecular biology applications
Description: HeLa Antigen Concentrate by Cygnus Technologies is available in Europe via Gentaur.

Hela Nuclear Extract

X12001 1000 µg Ask for price
Description: fast delivery possible

Total RNA from Human Tumor Tissue: Colon, sigmoid

R1235095-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Small Intestine

R1235226-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Membrane Protein from Human Tumor Cell Line: A431

P3255801 0.1 mg
EUR 285
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Membrane Protein from Human Tumor Cell Line: Jurkat

P3255815 0.1 mg
EUR 285
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Membrane Protein from Human Tumor Cell Line: Raji

P3255840 0.1 mg
EUR 285
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Horse TOTAL PROTEIN(TOTAL PROTEIN)

QY-E120081 96T
EUR 478

SKOV-3/Luc Cell Line

AKR-232 1 vial
EUR 572
Description: SKOV-3/Luc Cell Line stably expresses luciferase and otherwise exhibits the same characteristics of the parental cell line.

MCF-7/Luc Cell Line

AKR-234 1 vial
EUR 572
Description: MCF-7/Luc Cell Line stably expresses luciferase and otherwise exhibits the same characteristics of the parental cell line.

OVCAR-5/RFP Cell Line

AKR-254 1 vial
EUR 572
Description: OVCAR-5/RFP Cell Line stably expresses RFP and otherwise exhibits the same characteristics of the parental cell line.

OxiSelect Total Glutathione (GSSG/GSH) Assay Kit, Trial Size

STA-312-T 20 assays
EUR 282
Description: The OxiSelect Total Glutathione Assay Kit is a quantitative assay for measuring the total glutathione content within a sample (GSH/GSSG).  Glutathione Reductase reduces oxidized glutathione (GSSG) to reduced glutathione (GSH) in the presence of NADPH.  Subsequently, the  chromogen reacts with the thiol group of GSH to produce a colored compound that absorbs at 405 nm.  The total glutathione content in unknown samples is determined by comparison with the predetermined glutathione standard curve.  The rate of chromophore production is proportional to the concentration of glutathione within the sample.  The rate can be determined from the absorbance change over time.  Metaphosphoric acid is provided to remove interfering proteins or enzymes from samples.

OxiSelect Total Antioxidant Capacity (TAC) Assay Kit, Trial Size

STA-360-T 20 assays
EUR 282
Description: The OxiSelect Total Antioxidant Capacity (TAC) Assay measures the total antioxidant capacity of biomolecules from a variety of samples via a SET mechanism. In the presence of antioxidants, copper(II) is reduced to copper(I). In turn, the copper(I) ions react with a chromogen to produce a color with maximum absorbance at 490nm.

Matching Pair - Total Protein from Human Primary Tumor and Metastatic Tumor Tissue (PM): Breast

P8235086-PM 0.2 mg x2
EUR 1228
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Matching Pair - Total Protein from Human Primary Tumor and Metastatic Tumor Tissue (PM): Colon

P8235090-PM 0.2 mg x2
EUR 1228
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Matching Pair - Total Protein from Human Primary Tumor and Metastatic Tumor Tissue (PM): Kidney

P8235142-PM 0.2 mg x2
EUR 1228
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

The results presented here show here that the inactivation of the Jak-Stat signaling route significantly reduces the apoptotic response induced by infection, thus facilitating the establishment and maintenance of persistent IBDV infections. Members of the Birnaviridae family, including an infectious scholarship disease virus (IBDV). , has a double behavior, causing acute infections that are often followed by the establishment of long-service life asymptomatic infections. Indeed, persistently infected specimens could act as efficient virus tanks, so potentially contributing to the diffusion of viruses. Despite the key importance of this biological trait, information on mechanisms triggering IBDV persistence is negligible.

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