Background: Ms. malaria infection leads to poor perinatal outcomes, including low birth weight of preterm birth and / or fetal growth restriction, especially in primigravidae. In placental malaria, Plasmodium falciparum-infected red blood cells causing an inflammatory response that can interfere with maternal-fetal exchange, causing poor growth. Type I interferon (IFN-I) pathway plays an immunomodulatory role in viral and bacterial infections, usually by suppressing the inflammatory response. However, its role in placental malaria is unknown. This study examined the cytokine responses in placental tissues from a subset of malaria infected and uninfected women, and attempts to connect them with the results of certain birth.
Methods: 40 across the placental biopsy samples obtained from pregnant women at least 16 years were recruited to larger prospective chemoprevention trials against malaria. This is a patient in hospitals Tororo in Uganda, an area of high malaria endemicity where about 40% of women had evidence of malaria infection at delivery. They regularly attended at a local clinic and monitored for fever, blood smear done later and at the time of delivery for diagnosing malaria infection. Placental biopsies were taken for histological diagnosis of placental malaria, as well as quantitative PCR analysis of IFN-I genes in pathways (IFN-β, IL-10 and MX-1). Parameters such as the baby’s birth weight and gestational age were also recorded.
Results: Histological analysis revealed placental malaria on 18 samples, while 22 were found to be infected. RT-PCR analysis showed a fourfold increase in IFN-β and IL-10 expression in multigravidas with placental malaria compared with gravidity-matched, uninfected controls. This effect was not observed in primigravida. Interestingly, linear regression analysis showed a positive correlation between the levels of IFN-β and a higher birth weight (β = 101.2 g per log2-fold increase in the expression of IFN-β, p = 0.042). This association was strongest in primigravida with placental malaria (β = 339.0, p = 0.006).
Conclusion: These results suggest differential regulation of IFN-I pathway in placental malaria by gravidity, with the largest anti-inflammatory response seen in multigravidas. The relationship between the level of IFN-β and a higher birth weight also demonstrated a protective role for IFN-I on fetal growth restriction in placental malaria.
Gravidity-dependent associations between interferon response and birth weight in placental malaria
The role of cytotoxic T lymphocytes and interferon-γ coronavirus infection: lessons from a murine coronavirus infection in mice
Murine coronavirus (CoV) is a beta-CoV that infect mice by binding to antigen-related cell adhesion molecule-carcinoembryonic 1. intraperitoneal infection with murine CoV strain JHM (JHMV) induces mild acute hepatitis in mice. While both the innate and acquired immune responses play an important role in the protection against murine CoV infection in mice, CD8 + cytotoxic T lymphocytes (CTL) and interferon-γ is critical for the eradication of hepatitis virus-induced JHMV.
Rat Interferon-Stimulated 20 kDa Exonuclease-Like 2 (ISG20L2) ELISA Kit
Description: Recombinant Interferon gamma is a disulfide-linked monomer protein consisting of 135 amino acid residues, and migrates as an approximately 16 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding rat Interferon-gamma mature chain was expressed in E. coli.
Description: Recombinant Interferon gamma is a disulfide-linked monomer protein consisting of 135 amino acid residues, and migrates as an approximately 16 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding rat Interferon-gamma mature chain was expressed in E. coli.
Description: A competitive ELISA for quantitative measurement of Rat Interferon β in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Interferon β in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Interferon β in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Interferon β1a in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Interferon β1a in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Interferon β1a in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Rat Interferon ω1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Rat Interferon ω1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Rat Interferon ω1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Interferon β1b in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Interferon β1b in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Interferon β1b in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Rat Interferon alpha in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Rat Interferon alpha in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Rat Interferon alpha in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Interferon Alpha 21 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Interferon Alpha 21 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Interferon Alpha 21 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: Quantitativesandwich ELISA kit for measuring Rat Interferon γ , IFN-γ in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativesandwich ELISA kit for measuring Rat Interferon γ , IFN-γ in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: Quantitativesandwich ELISA kit for measuring Rat Interferon α, IFN-α in samples from serum, plasma, cell culture supernates, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativesandwich ELISA kit for measuring Rat Interferon α, IFN-α in samples from serum, plasma, cell culture supernates, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Additionally, CoVs characterized by a high diversity, caused by a mutation, recombination, and gene gain / loss. 25V16G is JHMV immune-escape variants, which do not have the dominant CTL epitope. By avoiding the immune response, 25V16G establish persistent infections, causing granulomatous serositis in mice interferon-γ-deficient. These examples pathogenesis-related CoV in mice may provide useful information about other CoV infection, including coronavirus disease in 2019 (COVID-19).
Ricardo
