Patients with grafted-counter-host diseases (GVHD) develop characteristic mucinous phenomena consisting of erosive erythema with histopathological results, including interface dermatitis and keratinocyte death, resulting in generous wastemate changes. We found that keratinocytes had a marked production of transforming growth factor (TGF) β1 in chronic GVHD cutaneous lesions, but not in acute GVHD. To further study keratinocyte roles, the main targets of donor cells, in changes in sclerodermata followed by interface dermatitis, we have established a murine model of chronic GVHD sclerodation changes, followed by an injury. GVHD acute muccutter in genetically transferred modified mice with T cells specific to keratinocytes. While the transfer of CD8 T8 T8 T8 cells deficient in Granzyme B has not resulted in a mucked injury or scleral changes in the recipients, the winners.
The recipients have developed a serious injury to acute mucocution, but changes in milder scleriferms compared to nature. Type the recipients of CD8 T cells. In addition, the rebate recipients CD8 TFNγ deficient have a lower expression of TGFβ1 in the epidermis than control. The primary murine keratinocytes undergoing apoptosis induced by FASL and incubated with IFNγ produce TGFβ1, whose production was inhibited by a Pan-Caspase inhibitor. Our results indicate that IFNγ promotes TGFβ1 production by apoptotic keratinocytes, giving media the development of generous scleral changes in Kératinocyte-targeting GVHD.
Tilapia lake Virus (TILV; Genre: Tilapinevirus, Family: AmnoOnviridae) is an enveloped virus recently characterized with a linear and negative one-way RNA genome, which causes high mortality in Tilapia species. In this study, we have demonstrated that zebra larvae (Danio Rerio) are sensitive to Tilv infection on a systemic injection. Tilv reproduced in fish-fish larvae and caused their high mortality (about 70%). Histopathological examination revealed that Tilv infection caused pathological anomalies in highly visible zebra fish larvae.
Lambda bovine interferon is a powerful antiviral against SARS-COV-2 in vitro infection
Interferon Lambda (IFN-λ) is an antiviral produced naturally in response to viral infections, with activity on epithelial cells and located on the mucosal surfaces. This localized activity leads to reduced toxicity with respect to Type I IFNs, whose receptors are ubiquitously expressed. IFN-λ has been effective in the therapy of respiratory viral infections, playing a crucial role in the potentiation of adaptive immune responses that initiate on mucosal surfaces. Human IFN-λ has polymorphisms that can cause differences in the interaction with the specific receiver of the human population.
Interestingly, the IFN-λ3 cattle has a higher silico affinity for the human receiver than its human counterparts, with a high identity with different human IFN-λ variants, making it a suitable antiviral therapeutic candidate for human health. Here, we demonstrate that a recombinant Cattle IFN-λ (RBIFN-λ) produced in Hek-293 cells is effective in preventing the SARS-COV-2 infection of Vero Cells, with a 50% inhibitory concentration ( IC50) between 30 and 50 times. lower than that of the human IFN IFN, tested here (α2b and β1a). We have also demonstrated the absence of RBIFN-λ toxicity in human PBMCs and lack of preflammatory activity on these cells.
In total, our results show that RBIFN-λ is like an effective antiviral potentially suitable for COVID-19 therapy. Among other potential applications, RBIFN-λ could be useful for preventing the dispersion of viruses on the lungs and / or to reduce the transmission of infected people. In addition, and because of the non-specific activity of this IFN, it can be potentially effective against other respiratory viruses that can circulate with SARS-COV-2.
Interferon-γ-stimulated apoptotic keratinocytes promote sclerodermatous changes in chronic graft-versus-host disease
Characteristics of the clinical, laboratory and interferon response of patients with chilblai type lesions during the CVIV-19 pandemic
Importance: Chilblain-like lesions have been reported at the 2019 Coronavirus Pandemic (COVID-19). Pathophysiology of such manifestations remains largely unknown.
Objective: To carry out a clinical, histological and biological assessment systematic in a cohort of patients with similar lesions to the Chilbain during the Covid-19 pandemic.
Design, framework and participants: In this series of forward-looking cases carried out with a CIVID-19 multidisciplinary consultation group at the Nice University Hospital, France, 40 consecutive patients with children similar to the Childland have been included.Key Results and Measures: Patients have undergone a general and dermatological general review, including skin biopses, vascular surveys, biological analyzes, interferon-alpha stimulation and detection (IFN-α) and acute respiratory syndrome. Courage Coronavirus 2 (SARS-COV-2) Polymerase chain reaction (PCR) and serological analysis.
Pig Interferon-stimulated gene 20 kDa protein ELISA Kit
Description: A competitive ELISA for quantitative measurement of Porcine Interferon β in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Interferon β in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Interferon β in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Porcine Interferon alpha in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Porcine Interferon alpha in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Porcine Interferon alpha in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Interferon β1b in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Interferon β1b in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Interferon β1b in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Interferon β1a in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Interferon β1a in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Interferon β1a in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Porcine Interferon ω1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Porcine Interferon ω1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Porcine Interferon ω1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Interferon Alpha 21 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Interferon Alpha 21 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Interferon Alpha 21 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Guinea pig Interferon β in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Guinea pig Interferon β in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Guinea pig Interferon β in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Interferon Beta (IFNb) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Interferon Beta (IFNb) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Interferon Beta (IFNb) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Interferon Beta (IFNb) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Pig Interferon Beta (IFNb) in samples from Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Pig Interferon Alpha (IFNa) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Pig Interferon Alpha (IFNa) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Pig Interferon Alpha (IFNa) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Pig Interferon Alpha (IFNa) in serum, plasma and other biological fluids.
Description: Double-antibody Sandwich chemiluminescent immunoassay for detection of Pig Interferon Alpha (IFNa)Serum, plasma and other biological fluids
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Pig Interferon Gamma (IFNg) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Pig Interferon Gamma (IFNg) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Pig Interferon Gamma (IFNg) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Pig Interferon Gamma (IFNg) in serum, plasma and other biological fluids.
Description: Double-antibody Sandwich chemiluminescent immunoassay for detection of Pig Interferon Gamma (IFNg)Serum, plasma and other biological fluids
Results: Overall, 40 consecutive patients with chuban-like lesions were included. Most patients were young, with a median era of 22 (12-67) years; 19 men and were women. The clinical presentation was highly reproducible with lesions similar to the chilblai on the toes. The bulleous and necrotic evolution was observed in 11 patients. Acroocyanosis or cold toes have been reported in 19 cases (47.5%). Criteria compatible with COVID-19 cases were noted in 11 (27.5%) within 6 weeks before the eruption. The results of real-time PCR tests (RT-PCR) were negative in all cases. Overall, the results of SARS-COV-2 serology were positive in 12 patients (30%). D-dimer concentration levels have been raised in 24 (60.0%). Cryoglobulinemia and Parvovirus B19 The serological results were negative for all patients tested. The main histological conclusions were characteristics of lymphocyte inflammation and vascular thickening damage to the Venula and hyperplasia of PERICYTE. A significant increase in IFN-α production after in vitro stimulation was observed in the population of Chilbignon compared to patients with slightly severe acute CVIV-19.
Ricardo
