Methylation regulation of Antiviral host factors, Interferon Stimulated Genes (ISGs) and T-cell responses associated with natural HIV control

Methylation regulation of Antiviral host factors, Interferon Stimulated Genes (ISGs) and T-cell responses associated with natural HIV control

GWAS analysis and screening immune biomarkers have been identified host factors associated with in vivo HIV-1 control. However, there are gaps in knowledge about the mechanisms that regulate the expression of host factors. Here, we aimed to assess DNA methylation affects the natural host genome in HIV-1 control. For this purpose, the entire DNA methylome in 70 HIV-1 infected individuals not treated with either high (> 50,000 HIV-1 RNA copies / ml, n = 29) or low (<10,000 HIV-1 RNA copies / ml, n = viral load (PVL) level 41) plasma compared and identified 2649 different positions methylated (DMP).

Of these, the forest classification model randomly selected 55 DMP were correlated with viral (PVL and proviral levels) and adaptive immunity parameters of HIV-1 specific (IFNg-T cell responses and neutralizing capacity of antibodies). Then, cluster and functional analysis identified two groups of DMP: cluster 1 contained the gene hypo-methylated involved in antiviral and interferon response (eg PARP9, MX1 and USP18) in individuals with high viral loads while in cluster 2, genes associated with T follicular helper cells (TFH) commitments (eg CXCR5 and TCF7) hyper-methylated in the same group of people with uncontrolled infection.

For selected genes, mRNA levels were negatively correlated with DNA methylation, which states the epigenetic regulation of gene expression. Furthermore, expression of this gene signature was also confirmed in the early stages of chronic infection, including untreated, treated and elite controllers ART HIV-1 infected individuals (n = 37). These data provide the first evidence that the critical host genes involved in the immune control of the virus is under the regulation of methylation in HIV-1 infection. This insight may offer a new opportunity to identify novel mechanisms in vivo control of the virus and may prove important for the development of future therapeutic interventions aimed at HIV-1 cure.

 Methylation regulation of Antiviral host factors, Interferon Stimulated Genes (ISGs) and T-cell responses associated with natural HIV control
Methylation regulation of Antiviral host factors, Interferon Stimulated Genes (ISGs) and T-cell responses associated with natural HIV control

Heat stress in the oocyte or zygote compromise embryonic development, production interferon tau damage and increases reactive oxygen species and oxidative stress in cattle embryos produced in vitro

Interferon tau (IFNT) is a cytokine that is responsible for the recognition of pregnant women in ruminants and play the role of embryo-maternal communication modulation in the oviduct induces local response of immune cells. We aimed to investigate the production of IFNT, reactive oxygen species and oxidative stress under the influence of heat stress (HS) during the different stages of cow in vitro embryo production.

HS was set up when the temperature is gradually raised from 38.5 ° C to 40.5 ° C in a laboratory incubator, sustained for 6 hours, and reduced back to 38.5 ° C. To overcome the effects of HS on IFNT production, reactive oxygen species, and oxidative stress, ovaries from a slaughterhouse that is used in accordance with the treatment: a control group (38.5 ° C); oocytes mature under the HS; fertilized oocytes under HS; zygotes were cultured on the first day under the HS; and delivered to the HS cell in oocyte maturation, fertilization and embryo culture first day.

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Description: This monoclonal antibody is suitable for detection of human IFN-γ using Western blot.

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Description: This monoclonal antibody enables sensitive and specific detection of human IFN-γ in immunoassays such as ELISA and ELISpot.

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Description: This monoclonal antibody enables sensitive and specific detection of human IFN-γ in immunoassays such as ELISA and ELISpot.

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Description: This ALP-conjugated monoclonal antibody enables sensitive and specific detection of human IFN-γ in ELISpot.

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  • Product category: Proteins/Recombinant Proteins/Cytokines

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Description: This monoclonal antibody enables sensitive and specific detection of rabbit IFN-gamma (IFN-γ) in immunoassays such as ELISA and ELISpot

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Description: This monoclonal antibody enables sensitive and specific detection of rabbit IFN-gamma (IFN-γ) in immunoassays such as ELISA, ELISpot and Flow cytometry.

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Description: This monoclonal antibody enables sensitive and specific detection of bovine IFN-γ in immunoassays such as ELISA, ELISpot and Flow cytometry.

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Description: This monoclonal antibody enables sensitive and specific detection of cat (feline) IFN-γ in immunoassays such as ELISA and ELISpot.

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Description: This monoclonal antibody enables sensitive and specific detection of cat (feline) IFN-γ in immunoassays such as ELISA and ELISpot.

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Description: IFN-gammaRalpha is a protein encoded by the IFNGR1 gene which is approximately 54,4 kDa. IFN-gammaRalpha is localised to the cell membrane and is involved in influenza A, Th17 cell differentiation, interferon gamma signalling, toxoplasmosis and type I interferon signalling pathways. It is a ligand-binding chain of the gamma interferon receptor which also includes IFNGR2. It associates with IFNGR2 to form a receptor for the cytokine interferon gamma. It is also associated with susceptibility to Helicobacter pylori infection. IFN-gammaRalpha is expressed in the nervous system, blood, spleen, lymph node and intestine. Mutations in the IFNGR1 gene result in immunodeficiency 27A and B. STJ93645 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of IFN-gammaRalpha protein.

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Description: Rabbit polyclonal to IFN gamma Receptor beta

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Description: Mouse monoclonal IFN gamma antibody

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Description: Rat monoclonal IFN gamma antibody

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Description: Mouse monoclonal IFN gamma antibody

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Description: Mouse monoclonal IFN gamma antibody

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Description: Rat monoclonal IFN gamma antibody

HS negatively affected the rate of cleavage and blastocyst, in all groups of HS. On Day 7, all HS-treated embryos showed a decrease IFNT gene and protein expression, whereas the reactive oxygen species increased compared with controls. In conclusion, early embryonic development is compromised because of the higher temperatures during in vitro oocyte maturation, fertilization, and / or the zygote stage has diminished IFNT expression and increased reactive oxygen species in cattle.

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