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STAT1 regulates interferon-γ-induced angiotensinogen and MCP-1 expression in a bidirectional manner in primary cultured mesangial cells
Objective : Intrarenal interferon-γ contributed significantly to the development of glomerular injury in which angiotensinogen and monocyte chemoattractant protein 1 levels were high. However, the exact nature of the role played by interferon-γ in regulating angiotensinogen and monocyte chemoattractant protein 1 expression has not been fully described. Therefore, the aim of this study was to investigate the role played by interferon-γ in angiotensinogen and monocyte chemoattractant protein 1 expression.
Methods: Primary cultured rat mesangial cells treated with 0-20 ng / mL interferon-γ for 2, 8 or 24 hours. angiotensinogen expression levels, monocyte chemoattractant protein 1, suppressor of cytokine signaling 1, suppressing the intracellular Janus kinase-signal transducer and activator of transcription signaling and activity of Janus kinase-signal transducer and activator of transcription pathway was evaluated by reverse transcriptase polymerase chain reaction and Western blot analysis.
Results: Interferon-γ increase the expression of angiotensinogen in mesangial cells was observed following the augmentation maximum 5 ng / mL interferon-γ at 8 hours of treatment (1.87 ± 0.05, mRNA, relative ratio). further increases reduced or no use of higher concentrations of interferon-γ. Following treatment, monocyte chemoattractant protein 1 expression is induced by a linear dose-dependent manner (6.85 ± 0.62-fold at 20 ng / mL interferon-γ at 24 hours). In addition, interferon-γ induced STAT1 phosphorylation and suppressor of cytokine signaling 1 expression with a linear dose-dependent manner.
Oppression STAT1 and suppressor of cytokine signaling 1 expression by small interference RNA angiotensinogen facilitated increased expression of interferon-γ-induced, suggesting that these two factors negatively regulate the expression of angiotensinogen. Conversely, an increase in interferon-γ-induced monocyte chemoattractant protein 1 expression was attenuated in STAT1-deficient mesangial cells, suggesting that STAT1 positively regulates monocyte chemoattractant protein 1 expression in mesangial cells.
Conclusion: These results indicate that while interferon-γ improve both angiotensinogen and monocyte chemoattractant protein 1 expression, STAT1 opponent played a role in the regulation of each of the factors in mesangial cells.
STAT1 regulates interferon-γ-induced angiotensinogen and MCP-1 expression in a bidirectional manner in primary cultured mesangial cells
Interferon and virus induces novel primate-specific isoform dACE2 and not SARS-CoV-2 receptor ACE2
acute respiratory syndrome-2 coronavirus (SARS-CoV-2), which causes COVID-19, take advantage of the angiotensin-converting enzyme 2 (ACE2) to enter the target cells. ACE2 has been proposed as interferon-stimulated genes (ISG). Thus, the interferon-induced variability in the level of ACE2 expression may be important for susceptibility to COVID-19 or its results. Here, we report the discovery of a new isoform, primate-specific ACE2, which we refer to as deltaACE2 (dACE2).
We show that dACE2, but not ACE2, the ISG. In vitro, dACE2, which has 356 amino acid N-terminal, is non-functional in binding of SARS-CoV spike protein-2 and as Karboksipeptidase A. Our results reconcile the current knowledge on the expression of ACE2 and suggested that the ISG-type induction dACE2 IFN-high in the conditions created by the treatment, the inflammatory tumor microenvironment, or virus co-infection is unlikely to affect the cellular influx of SARS-CoV-2 and promote infection.
Human CellExp? Human CD40/ TNFRSF5, Human recombinant
9230-10
Biovision
EUR 333.6
Human CellExp? Human CD40/ TNFRSF5, Human recombinant
9230-50
Biovision
EUR 868.8
Human CellExp? Renin, Human Recombinant
6300-10
Biovision
EUR 196.8
Human CellExp? Renin, Human Recombinant
6300-100
Biovision
EUR 940.8
Human CellExp? Renin, Human Recombinant
6300-50
Biovision
EUR 601.2
Human CellExp? EPO, Human Recombinant
6447-10
Biovision
EUR 646.8
Human CellExp? EPO, Human Recombinant
6447-50
Biovision
EUR 2017.2
Human CellExp? HGF, Human Recombinant
6456-10
Biovision
EUR 464.4
Human CellExp? HGF, Human Recombinant
6456-50
Biovision
EUR 1677.6
Human CellExp? HGH, Human Recombinant
6457-10
Biovision
EUR 405.6
Human CellExp? HGH, Human Recombinant
6457-50
Biovision
EUR 1455.6
Human CellExp? Noggin, Human Recombinant
6474-10
Biovision
EUR 360
Human CellExp? Noggin, Human Recombinant
6474-50
Biovision
EUR 1286.4
Human CellExp? SCF, Human Recombinant
6478-10
Biovision
EUR 411.6
Human CellExp? SCF, Human Recombinant
6478-50
Biovision
EUR 1599.6
Human CellExp? TPO, Human Recombinant
6483-10
Biovision
EUR 438
Human CellExp? TPO, Human Recombinant
6483-50
Biovision
EUR 2376
Human CellExp? Progranulin, Human Recombinant
4738-10
Biovision
EUR 366
Human CellExp? Progranulin, Human Recombinant
4738-100
Biovision
EUR 2727.6
Human CellExp? TIGIT, human recombinant
8015-10
Biovision
EUR 418.8
Human CellExp? Azurocidin, human recombinant
8017-20
Biovision
EUR 320.4
Human CellExp? CD163, Human recombinant
9226-10
Biovision
EUR 366
Human CellExp? CD163, Human recombinant
9226-50
Biovision
EUR 927.6
Human CellExp? BTLA, Human recombinant
9228-10
Biovision
EUR 346.8
Human CellExp? BTLA, Human recombinant
9228-50
Biovision
EUR 882
Human CellExp? CD28, Human recombinant
9237-10
Biovision
EUR 268.8
Human CellExp? CD28, Human recombinant
9237-100
Biovision
EUR 868.8
Human CellExp? CD19, Human recombinant
9246-10
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EUR 320.4
Human CellExp? CD19, Human recombinant
9246-25
Biovision
EUR 548.4
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7122-10
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EUR 170.4
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7122-50
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EUR 430.8
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7215-10
Biovision
EUR 288
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7215-50
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EUR 1032
Human CellExp? BAFF, human recombinant
7222-10
Biovision
EUR 307.2
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7223-10
Biovision
EUR 255.6
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7228-10
Biovision
EUR 352.8
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7239-10
Biovision
EUR 307.2
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7243-50
Biovision
EUR 411.6
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7247-10
Biovision
EUR 470.4
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7247-50
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EUR 1396.8
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7248-10
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EUR 398.4
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7248-50
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EUR 1324.8
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7249-10
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EUR 548.4
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7250-10
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EUR 379.2
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7265-20
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7267-10
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EUR 464.4
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7371-10
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EUR 294
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7371-50
Biovision
EUR 836.4
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7383-10
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EUR 280.8
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7383-50
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EUR 810
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7385-10
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7394-10
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7411-10
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7412-10
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7412-50
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EUR 836.4
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7435-10
Biovision
EUR 314.4
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7435-50
Biovision
EUR 861.6
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7440-10
Biovision
EUR 366
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7451-100
Biovision
EUR 861.6
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7451-20
Biovision
EUR 326.4
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7453-10
Biovision
EUR 339.6
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7455-10
Biovision
EUR 294
Human CellExp? CD147, human recombinant
7455-50
Biovision
EUR 836.4
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7462-10
Biovision
EUR 294
Human CellExp? CD155, human recombinant
7462-50
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EUR 836.4
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7464-10
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7464-50
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EUR 836.4
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7465-10
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7465-50
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7467-100
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7467-20
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7471-10
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7471-50
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EUR 802.8
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7472-10
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7472-50
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EUR 992.4
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7481-10
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EUR 360
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7563-10
Biovision
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7563-50
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EUR 1038
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7576-10
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EUR 326.4
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7834-100
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7834-20
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P1014-10
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P1014-50
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P1015-10
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P1015-25
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P1018-10
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EUR 248.4
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P1018-50
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EUR 574.8
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P1020-10
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EUR 210
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P1022-10
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P1022-25
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P1082-10
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P1084-10
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EUR 183.6
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P1084-50
Biovision
EUR 639.6
Human CellExp? CD83, Human recombinant
P1109-10
Biovision
EUR 157.2
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P1109-50
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EUR 483.6
Human CellExp? CD160, human recombinant
P1110-10
Biovision
EUR 170.4
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P1110-50
Biovision
EUR 470.4
Human CellExp? CNDP1, Human recombinant
P1125-10
Biovision
EUR 248.4
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P1125-50
Biovision
EUR 1005.6
Human CellExp? VAPB, Human recombinant
P1135-10
Biovision
EUR 157.2
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P1135-50
Biovision
EUR 444
Human CellExp? Transthyretin, Human recombinant
P1138-20
Biovision
EUR 222
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P1138-50
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EUR 470.4
Human CellExp? Sclerostin, human recombinant
P1145-10
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EUR 183.6
Human CellExp? Sclerostin, human recombinant
P1145-50
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EUR 639.6
Human CellExp? SLITRK6, human recombinant
P1146-10
Biovision
EUR 170.4
Human CellExp? SLITRK6, human recombinant
P1146-50
Biovision
EUR 535.2
Human CellExp? LILRB2, human recombinant
P1147-10
Biovision
EUR 157.2
Human CellExp? LILRB2, human recombinant
P1147-50
Biovision
EUR 483.6
Human CellExp? ROR1, human recombinant
P1152-10
Biovision
EUR 170.4
Human CellExp? ROR1, human recombinant
P1152-50
Biovision
EUR 535.2
Human CellExp? DLL3, Human Recombinant
P1165-10
Biovision
EUR 679.2
Human CellExp? DLK1, Human Recombinant
P1166-10
Biovision
EUR 666
Human CellExp? PTPRD, Human Recombinant
P1184-10
Biovision
EUR 183.6
Human CellExp? PTPRD, Human Recombinant
P1184-50
Biovision
EUR 614.4
Human CellExp? KRAS, Human Recombinant
P1198-10
Biovision
EUR 170.4
Human CellExp? KRAS, Human Recombinant
P1198-1000
Biovision
EUR 3184.8
Human CellExp? KRAS, Human Recombinant
P1198-250
Biovision
EUR 1070.4
Human CellExp? KRAS, Human Recombinant
P1198-50
Biovision
EUR 314.4
Human CellExp? Hemopexin, Human Recombinant
P1210-10
Biovision
EUR 235.2
Human CellExp? Hemopexin, Human Recombinant
P1210-50
Biovision
EUR 796.8
Human CellExp? ECE1, Human Recombinant
P1227-10
Biovision
EUR 301.2
Human CellExp? ECE1, Human Recombinant
P1227-50
Biovision
EUR 1214.4
Human CellExp? CD8b, Human Recombinant
P1244-10
Biovision
EUR 288
Human CellExp? CD8b, Human Recombinant
P1244-1000
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Human CellExp? CD8b, Human Recombinant
P1244-250
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Human CellExp? CD8b, Human Recombinant
P1244-50
Biovision
EUR 966
Human CellExp? ROR2, Human Recombinant
P1254-10
Biovision
EUR 274.8
Human CellExp? VEGF110, Human Recombinant
P1258-10
Biovision
EUR 574.8
Human CellExp? Prolactin, Human Recombinant
P1311-10
Biovision
EUR 183.6
Human CellExp? Prolactin, Human Recombinant
P1311-50
Biovision
EUR 288
Human CellExp? Dermatopontin, Human Recombinant
P1319-10
Biovision
EUR 405.6
Human CellExp? CD177, Human Recombinant
P1410-10
Biovision
EUR 183.6
Human CellExp? CD177, Human Recombinant
P1410-50
Biovision
EUR 470.4
Human CellExp? PM20D1, Human Recombinant
P1430-10
Biovision
EUR 483.6
Human CellExp? DLL3, Human Recombinant
P1463-10
Biovision
EUR 202.8
Human CellExp? DLL3, Human Recombinant
P1463-50
Biovision
EUR 601.2
Human CellExp? Properdin, Human Recombinant
P1470-10
Biovision
EUR 196.8
Human CellExp? Properdin, Human Recombinant
P1470-50
Biovision
EUR 574.8
Human CellExp? Activin A, Human Recombinant
6442-10
Biovision
EUR 802.8
Human CellExp? Activin A, Human Recombinant
6442-50
Biovision
EUR 2526
Human CellExp? BMP-2, Human Recombinant
6444-10
Biovision
EUR 502.8
Human CellExp? BMP-2, Human Recombinant
6444-50
Biovision
EUR 1677.6
Human CellExp? BMP-4, Human Recombinant
6445-10
Biovision
EUR 457.2
Human CellExp? BMP-4, Human Recombinant
6445-50
Biovision
EUR 1599.6
Human CellExp? G-CSF, Human Recombinant
6453-10
Biovision
EUR 496.8
Human CellExp? G-CSF, Human Recombinant
6453-50
Biovision
EUR 1677.6
Human CellExp? GM-CSF, Human Recombinant
6454-10
Biovision
EUR 411.6
Human CellExp? GM-CSF, Human Recombinant
6454-50
Biovision
EUR 1599.6
Human CellExp? M-CSF, Human Recombinant
6455-10
Biovision
EUR 489.6
Human CellExp? M-CSF, Human Recombinant
6455-1000
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Human CellExp? M-CSF, Human Recombinant
6455-250
Biovision
EUR 6225.6
Human CellExp? M-CSF, Human Recombinant
6455-50
Biovision
EUR 1645.2
Human CellExp? IL-1beta, Human Recombinant
6460-10
Biovision
EUR 516
Human CellExp? IL-1beta, Human Recombinant
6460-50
Biovision
EUR 1677.6
Human CellExp? IL-2, Human Recombinant
6461-10
Biovision
EUR 320.4
Human CellExp? IL-2, Human Recombinant
6461-50
Biovision
EUR 946.8
Human CellExp? IL-3, Human Recombinant
6462-10
Biovision
EUR 457.2
Human CellExp? IL-3, Human Recombinant
6462-50
Biovision
EUR 1599.6
Human CellExp? IL-4, Human Recombinant
6463-10
Biovision
EUR 477.6
Human CellExp? IL-4, Human Recombinant
6463-50
Biovision
EUR 1677.6
Human CellExp? IL-6, Human Recombinant
6464-10
Biovision
EUR 274.8
Human CellExp? IL-6, Human Recombinant
6464-1000
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Human CellExp? IL-6, Human Recombinant
6464-250
Biovision
EUR 3276
Human CellExp? IL-6, Human Recombinant
6464-50
Biovision
EUR 861.6
Human CellExp? IL-10, Human Recombinant
6466-10
Biovision
EUR 411.6
Human CellExp? IL-10, Human Recombinant
6466-50
Biovision
EUR 1599.6
Human CellExp? IL-12, Human Recombinant
6467-10
Biovision
EUR 411.6
Human CellExp? IL-12, Human Recombinant
6467-50
Biovision
EUR 1599.6
Human CellExp? IL-17A, Human Recombinant
6468-10
Biovision
EUR 235.2
Human CellExp? IL-17A, Human Recombinant
6468-50
Biovision
EUR 764.4
Human CellExp? IL-17F, Human Recombinant
6469-10
Biovision
EUR 235.2
Human CellExp? IL-17F, Human Recombinant
6469-50
Biovision
EUR 764.4
Human CellExp? IL-28B, Human Recombinant
6472-10
Biovision
EUR 411.6
Human CellExp? IL-28B, Human Recombinant
6472-50
Biovision
EUR 1599.6
Human CellExp? IL-29, Human Recombinant
6473-10
Biovision
EUR 411.6
Human CellExp? IL-29, Human Recombinant
6473-50
Biovision
EUR 1599.6
Human CellExp? Oncostatin M, Human Recombinant
6475-10
Biovision
EUR 457.2
Human CellExp? Oncostatin M, Human Recombinant
6475-50
Biovision
EUR 1749.6
Human CellExp? TNF-alpha, Human Recombinant
6482-10
Biovision
EUR 235.2
Human CellExp? TNF-alpha, Human Recombinant
6482-50
Biovision
EUR 764.4
Human CellExp? VEGF 121, Human Recombinant
6484-10
Biovision
EUR 411.6
Human CellExp? VEGF 121, Human Recombinant
6484-50
Biovision
EUR 1599.6
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6485-10
Biovision
EUR 360
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6485-1000
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Human CellExp? VEGF 165, Human Recombinant
6485-50
Biovision
EUR 1383.6
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4737-10
Biovision
EUR 392.4
Development and research into new therapies that selectively and effectively destroy tumor cells that overexpress ERBB2 receptor is a pressing task. More recently, research into the use of type I interferon in the treatment of cancer has been intensified.