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STAT1 regulates interferon-γ-induced angiotensinogen and MCP-1 expression in a bidirectional manner in primary cultured mesangial cells
Objective : Intrarenal interferon-γ contributed significantly to the development of glomerular injury in which angiotensinogen and monocyte chemoattractant protein 1 levels were high. However, the exact nature of the role played by interferon-γ in regulating angiotensinogen and monocyte chemoattractant protein 1 expression has not been fully described. Therefore, the aim of this study was to investigate the role played by interferon-γ in angiotensinogen and monocyte chemoattractant protein 1 expression.
Methods: Primary cultured rat mesangial cells treated with 0-20 ng / mL interferon-γ for 2, 8 or 24 hours. angiotensinogen expression levels, monocyte chemoattractant protein 1, suppressor of cytokine signaling 1, suppressing the intracellular Janus kinase-signal transducer and activator of transcription signaling and activity of Janus kinase-signal transducer and activator of transcription pathway was evaluated by reverse transcriptase polymerase chain reaction and Western blot analysis.
Results: Interferon-γ increase the expression of angiotensinogen in mesangial cells was observed following the augmentation maximum 5 ng / mL interferon-γ at 8 hours of treatment (1.87 ± 0.05, mRNA, relative ratio). further increases reduced or no use of higher concentrations of interferon-γ. Following treatment, monocyte chemoattractant protein 1 expression is induced by a linear dose-dependent manner (6.85 ± 0.62-fold at 20 ng / mL interferon-γ at 24 hours). In addition, interferon-γ induced STAT1 phosphorylation and suppressor of cytokine signaling 1 expression with a linear dose-dependent manner.
Oppression STAT1 and suppressor of cytokine signaling 1 expression by small interference RNA angiotensinogen facilitated increased expression of interferon-γ-induced, suggesting that these two factors negatively regulate the expression of angiotensinogen. Conversely, an increase in interferon-γ-induced monocyte chemoattractant protein 1 expression was attenuated in STAT1-deficient mesangial cells, suggesting that STAT1 positively regulates monocyte chemoattractant protein 1 expression in mesangial cells.
Conclusion: These results indicate that while interferon-γ improve both angiotensinogen and monocyte chemoattractant protein 1 expression, STAT1 opponent played a role in the regulation of each of the factors in mesangial cells.
STAT1 regulates interferon-γ-induced angiotensinogen and MCP-1 expression in a bidirectional manner in primary cultured mesangial cells
Interferon and virus induces novel primate-specific isoform dACE2 and not SARS-CoV-2 receptor ACE2
acute respiratory syndrome-2 coronavirus (SARS-CoV-2), which causes COVID-19, take advantage of the angiotensin-converting enzyme 2 (ACE2) to enter the target cells. ACE2 has been proposed as interferon-stimulated genes (ISG). Thus, the interferon-induced variability in the level of ACE2 expression may be important for susceptibility to COVID-19 or its results. Here, we report the discovery of a new isoform, primate-specific ACE2, which we refer to as deltaACE2 (dACE2).
We show that dACE2, but not ACE2, the ISG. In vitro, dACE2, which has 356 amino acid N-terminal, is non-functional in binding of SARS-CoV spike protein-2 and as Karboksipeptidase A. Our results reconcile the current knowledge on the expression of ACE2 and suggested that the ISG-type induction dACE2 IFN-high in the conditions created by the treatment, the inflammatory tumor microenvironment, or virus co-infection is unlikely to affect the cellular influx of SARS-CoV-2 and promote infection.
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Human CellExp? Thrombomodulin, Human Recombinant
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Human CellExp? ADAM12, human recombinant
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Biovision
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Human CellExp? DKK3, human recombinant
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Biovision
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Human CellExp? SPAM1, human recombinant
7247-10
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Human CellExp? SPAM1, human recombinant
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Biovision
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Human CellExp? CD36, human recombinant
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Human CellExp? CD80, human recombinant
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Biovision
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Human CellExp? TNFRII, human recombinant
7394-10
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Human CellExp? SERPIND1, human recombinant
7411-10
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Human CellExp? VTCN1, human recombinant
7412-10
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Human CellExp? VTCN1, human recombinant
7412-50
Biovision
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Biovision
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7451-100
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Human CellExp? EPHB4, human recombinant
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7453-10
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7455-10
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Human CellExp? CD147, human recombinant
7455-50
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Human CellExp? FOLR1, human recombinant
7456-10
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Human CellExp? FOLR1, human recombinant
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Biovision
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7462-10
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7462-50
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7464-10
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7465-10
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7465-50
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7471-10
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7481-10
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7563-10
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7563-50
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7576-10
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7834-100
Biovision
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8015-10
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8017-20
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9226-10
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9226-50
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9228-10
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9228-50
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9237-10
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9237-100
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9246-10
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9246-25
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4738-10
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6300-10
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6300-100
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6447-10
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6456-10
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6457-10
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6474-10
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6478-10
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6483-10
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Human CellExp? VEGF110, Human Recombinant
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Human CellExp? CD177, Human Recombinant
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Human CellExp? CD177, Human Recombinant
P1410-50
Biovision
EUR 392
Human CellExp? PM20D1, Human Recombinant
P1430-10
Biovision
EUR 403
Human CellExp? DLL3, Human Recombinant
P1463-10
Biovision
EUR 169
Human CellExp? DLL3, Human Recombinant
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Biovision
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Human CellExp? Properdin, Human Recombinant
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Biovision
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Human CellExp? Properdin, Human Recombinant
P1470-50
Biovision
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Human CellExp? CD38, human recombinant
P1014-10
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Human CellExp? CD38, human recombinant
P1014-50
Biovision
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Human CellExp? CD45, human recombinant
P1015-10
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Human CellExp? CD45, human recombinant
P1015-25
Biovision
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Human CellExp? VSIG4, human recombinant
P1018-10
Biovision
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Human CellExp? VSIG4, human recombinant
P1018-50
Biovision
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Human CellExp? TrkA, human recombinant
P1020-10
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Human CellExp? NOV, human recombinant
P1022-10
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P1022-25
Biovision
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Human CellExp? EGFRvIII, human recombinant
P1082-10
Biovision
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Human CellExp? LOXL2, human recombinant
P1084-10
Biovision
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Human CellExp? LOXL2, human recombinant
P1084-50
Biovision
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Human CellExp? CD83, Human recombinant
P1109-10
Biovision
EUR 131
Human CellExp? CD83, Human recombinant
P1109-50
Biovision
EUR 403
Human CellExp? CD160, human recombinant
P1110-10
Biovision
EUR 142
Human CellExp? CD160, human recombinant
P1110-50
Biovision
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Human CellExp? CNDP1, Human recombinant
P1125-10
Biovision
EUR 207
Human CellExp? CNDP1, Human recombinant
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Biovision
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Human CellExp? IL-34, Human Recombinant
7108-10
Biovision
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Human CellExp? IL-34, Human Recombinant
7108-50
Biovision
EUR 1132
Human CellExp? DKK-1, Human Recombinant
7132-10
Biovision
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Human CellExp? DKK-1, Human Recombinant
7132-50
Biovision
EUR 1132
Human CellExp? sDLL-1, Human Recombinant
7133-10
Biovision
EUR 207
Human CellExp? sDLL-1, Human Recombinant
7133-50
Biovision
EUR 675
Human CellExp? sDLL-4, Human Recombinant
7134-10
Biovision
EUR 207
Human CellExp? sDLL-4, Human Recombinant
7134-50
Biovision
EUR 675
Human CellExp? PECAM-1, Human Recombinant
7184-10
Biovision
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Human CellExp? PECAM-1, Human Recombinant
7184-50
Biovision
EUR 381
Human CellExp? VCAM-1, Human Recombinant
7211-10
Biovision
EUR 142
Human CellExp? VCAM-1, Human Recombinant
7211-50
Biovision
EUR 381
Human CellExp? TLR-3, Human Recombinant
7220-10
Biovision
EUR 207
Human CellExp? TLR-3, Human Recombinant
7220-50
Biovision
EUR 675
Human CellExp? IL-17E, human recombinant
7224-10
Biovision
EUR 370
Human CellExp? VEGF-B, human recombinant
7230-10
Biovision
EUR 370
Human CellExp? VEGF-C, human recombinant
7231-10
Biovision
EUR 370
Human CellExp? Granzyme B, human recombinant
7233-10
Biovision
EUR 555
Human CellExp? SERPINA1 /A1AT, human recombinant
7235-10
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EUR 305
Human CellExp? SERPIN A8, human recombinant
7236-10
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EUR 316
Human CellExp? HMGB1 /HMG1, human recombinant
7240-10
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EUR 229
Human CellExp? HMGB1 /HMG1, human recombinant
7240-50
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EUR 582
Human CellExp? MMP-1, human recombinant
7244-10
Biovision
EUR 316
Human CellExp? MMP-1, human recombinant
7244-50
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EUR 1050
Human CellExp? MMP-2, human recombinant
7245-10
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EUR 332
Human CellExp? MMP-2, human recombinant
7245-50
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7246-10
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7246-1000
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7246-250
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EUR 3878
Human CellExp? MMP-9, human recombinant
7246-50
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EUR 1137
Human CellExp? IFN-gamma, human recombinant
7271-10
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EUR 256
Human CellExp? IL-22, human recombinant
7272-10
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EUR 359
Human CellExp? IL-15, human recombinant
7273-10
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EUR 512
Human CellExp? IL-7, human recombinant
7274-10
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Human CellExp? PDGF-BB, human recombinant
7276-10
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Human CellExp? Cathepsin S, human recombinant
7277-10
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EUR 349
Human CellExp? Cathepsin S, human recombinant
7277-50
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Human CellExp?LAG3 /CD223, human recombinant
7278-10
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EUR 267
Human CellExp?LAG3 /CD223, human recombinant
7278-50
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Human CellExp? CD360 / IL21R, human recombinant
7373-100
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Human CellExp? CD360 / IL21R, human recombinant
7373-20
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EUR 245
Human CellExp? CD306 / LAIR2, human recombinant
7377-10
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Human CellExp? CD306 / LAIR2, human recombinant
7377-50
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Human CellExp? CD84 / SLAMF5, human recombinant
7378-10
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7378-50
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Human CellExp? LAMP1/CD107a, human recombinant
7381-10
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7381-50
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Human CellExp? CD74/DHLAG, human recombinant
7387-10
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Human CellExp? CD74/DHLAG, human recombinant
7387-50
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Development and research into new therapies that selectively and effectively destroy tumor cells that overexpress ERBB2 receptor is a pressing task. More recently, research into the use of type I interferon in the treatment of cancer has been intensified.