STAT1 regulates interferon-γ-induced angiotensinogen and MCP-1 expression in a bidirectional manner in primary cultured mesangial cells

STAT1 regulates interferon-γ-induced angiotensinogen and MCP-1 expression in a bidirectional manner in primary cultured mesangial cells

Objective: Intrarenal interferon-γ contributed significantly to the development of glomerular injury in which angiotensinogen and monocyte chemoattractant protein 1 levels were high. However, the exact nature of the role played by interferon-γ in regulating angiotensinogen and monocyte chemoattractant protein 1 expression has not been fully described. Therefore, the aim of this study was to investigate the role played by interferon-γ in angiotensinogen and monocyte chemoattractant protein 1 expression.


Methods: Primary cultured rat mesangial cells treated with 0-20 ng / mL interferon-γ for 2, 8 or 24 hours. angiotensinogen expression levels, monocyte chemoattractant protein 1, suppressor of cytokine signaling 1, suppressing the intracellular Janus kinase-signal transducer and activator of transcription signaling and activity of Janus kinase-signal transducer and activator of transcription pathway was evaluated by reverse transcriptase polymerase chain reaction and Western blot analysis.


Results:
Interferon-γ increase the expression of angiotensinogen in mesangial cells was observed following the augmentation maximum 5 ng / mL interferon-γ at 8 hours of treatment (1.87 ± 0.05, mRNA, relative ratio). further increases reduced or no use of higher concentrations of interferon-γ. Following treatment, monocyte chemoattractant protein 1 expression is induced by a linear dose-dependent manner (6.85 ± 0.62-fold at 20 ng / mL interferon-γ at 24 hours). In addition, interferon-γ induced STAT1 phosphorylation and suppressor of cytokine signaling 1 expression with a linear dose-dependent manner.

Oppression STAT1 and suppressor of cytokine signaling 1 expression by small interference RNA angiotensinogen facilitated increased expression of interferon-γ-induced, suggesting that these two factors negatively regulate the expression of angiotensinogen. Conversely, an increase in interferon-γ-induced monocyte chemoattractant protein 1 expression was attenuated in STAT1-deficient mesangial cells, suggesting that STAT1 positively regulates monocyte chemoattractant protein 1 expression in mesangial cells.


Conclusion: These results indicate that while interferon-γ improve both angiotensinogen and monocyte chemoattractant protein 1 expression, STAT1 opponent played a role in the regulation of each of the factors in mesangial cells.

STAT1 regulates interferon-γ-induced angiotensinogen and MCP-1 expression in a bidirectional manner in primary cultured mesangial cells
STAT1 regulates interferon-γ-induced angiotensinogen and MCP-1 expression in a bidirectional manner in primary cultured mesangial cells

Interferon and virus induces novel primate-specific isoform dACE2 and not SARS-CoV-2 receptor ACE2

acute respiratory syndrome-2 coronavirus (SARS-CoV-2), which causes COVID-19, take advantage of the angiotensin-converting enzyme 2 (ACE2) to enter the target cells. ACE2 has been proposed as interferon-stimulated genes (ISG). Thus, the interferon-induced variability in the level of ACE2 expression may be important for susceptibility to COVID-19 or its results. Here, we report the discovery of a new isoform, primate-specific ACE2, which we refer to as deltaACE2 (dACE2).

We show that dACE2, but not ACE2, the ISG. In vitro, dACE2, which has 356 amino acid N-terminal, is non-functional in binding of SARS-CoV spike protein-2 and as Karboksipeptidase A. Our results reconcile the current knowledge on the expression of ACE2 and suggested that the ISG-type induction dACE2 IFN-high in the conditions created by the treatment, the inflammatory tumor microenvironment, or virus co-infection is unlikely to affect the cellular influx of SARS-CoV-2 and promote infection.

Human CellExp? Progranulin, Human Recombinant

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EUR 305

Human CellExp? Progranulin, Human Recombinant

4738-100
EUR 2273

Human CellExp? Renin, Human Recombinant

6300-10
EUR 164

Human CellExp? Renin, Human Recombinant

6300-100
EUR 784

Human CellExp? Renin, Human Recombinant

6300-50
EUR 501

Human CellExp? EPO, Human Recombinant

6447-10
EUR 539

Human CellExp? EPO, Human Recombinant

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EUR 1681

Human CellExp? HGF, Human Recombinant

6456-10
EUR 387

Human CellExp? HGF, Human Recombinant

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EUR 1398

Human CellExp? HGH, Human Recombinant

6457-10
EUR 338

Human CellExp? HGH, Human Recombinant

6457-50
EUR 1213

Human CellExp? Noggin, Human Recombinant

6474-10
EUR 300

Human CellExp? Noggin, Human Recombinant

6474-50
EUR 1072

Human CellExp? SCF, Human Recombinant

6478-10
EUR 343

Human CellExp? SCF, Human Recombinant

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EUR 1333

Human CellExp? TPO, Human Recombinant

6483-10
EUR 365

Human CellExp? TPO, Human Recombinant

6483-50
EUR 1980

Human CellExp? sCD14, Human Recombinant

7122-10
EUR 142

Human CellExp? sCD14, Human Recombinant

7122-50
EUR 359

Human CellExp? Thrombomodulin, Human Recombinant

7215-10
EUR 240

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7215-50
EUR 860

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7222-10
EUR 256

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7223-10
EUR 213

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7225-10
EUR 278

Human CellExp? EGF, human recombinant

7228-10
EUR 294

Human CellExp? ADAM12, human recombinant

7239-10
EUR 256

Human CellExp? DKK3, human recombinant

7243-50
EUR 343

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7247-10
EUR 392

Human CellExp? SPAM1, human recombinant

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EUR 1164

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EUR 316

Human CellExp? PCSK9, human recombinant

7265-20
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7267-10
EUR 387

Human CellExp? CD36, human recombinant

7371-10
EUR 245

Human CellExp? CD36, human recombinant

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EUR 697

Human CellExp? CD80, human recombinant

7383-10
EUR 234

Human CellExp? CD80, human recombinant

7383-50
EUR 675

Human CellExp? CD47, human recombinant

7385-10
EUR 278

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7394-10
EUR 256

Human CellExp? SERPIND1, human recombinant

7411-10
EUR 289

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Human CellExp? VTCN1, human recombinant

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EUR 697

Human CellExp? CD23, human recombinant

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EUR 262

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EUR 718

Human CellExp? TIMP1, human recombinant

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EUR 305

Human CellExp? EPHB4, human recombinant

7451-100
EUR 718

Human CellExp? EPHB4, human recombinant

7451-20
EUR 272

Human CellExp? TFPI, human recombinant

7453-10
EUR 283

Human CellExp? CD147, human recombinant

7455-10
EUR 245

Human CellExp? CD147, human recombinant

7455-50
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Human CellExp? FOLR1, human recombinant

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Human CellExp? FOLR1, human recombinant

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EUR 729

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EUR 278

Human CellExp? FOLR2, human recombinant

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EUR 234

Human CellExp? FOLR2, human recombinant

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EUR 669

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EUR 865

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EUR 272

Human CellExp?CD4, human recombinant

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EUR 267

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EUR 446

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Human CellExp? LILRB2, human recombinant

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EUR 403

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EUR 142

Human CellExp? ROR1, human recombinant

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EUR 446

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EUR 566

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EUR 664

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EUR 164

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EUR 479

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EUR 207

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EUR 479

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EUR 251

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EUR 457

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EUR 207

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EUR 479

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P1020-10
EUR 175

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P1022-10
EUR 196

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P1022-25
EUR 294

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EUR 283

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EUR 153

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P1084-50
EUR 533

Human CellExp? CD83, Human recombinant

P1109-10
EUR 131

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EUR 403

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P1110-10
EUR 142

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P1110-50
EUR 392

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P1125-10
EUR 207

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P1125-50
EUR 838

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4737-10
EUR 327

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EUR 930

Human CellExp? Activin A, Human Recombinant

6442-10
EUR 669

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6442-50
EUR 2105

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6443-10
EUR 283

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6443-50
EUR 843

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EUR 419

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EUR 1398

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EUR 381

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EUR 1333

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6446-10
EUR 419

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6446-50
EUR 1398

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6449-10
EUR 283

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6449-50
EUR 903

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6451-10
EUR 283

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EUR 849

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6453-10
EUR 414

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EUR 1398

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EUR 343

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6455-10
EUR 408

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6455-250
EUR 5188

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EUR 1371

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6460-10
EUR 430

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6460-50
EUR 1398

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6461-10
EUR 267

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EUR 789

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EUR 381

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6463-10
EUR 398

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EUR 229

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EUR 2730

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EUR 718

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EUR 343

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EUR 1333

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6466-10
EUR 343

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6466-50
EUR 1333

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6467-10
EUR 343

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6467-50
EUR 1333

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6468-10
EUR 196

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EUR 637

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6469-10
EUR 196

Human CellExp? IL-17F, Human Recombinant

6469-50
EUR 637

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6470-10
EUR 479


Development and research into new therapies that selectively and effectively destroy tumor cells that overexpress ERBB2 receptor is a pressing task. More recently, research into the use of type I interferon in the treatment of cancer has been intensified.

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