Objective: Intrarenal interferon-γ contributed significantly to the development of glomerular injury in which angiotensinogen and monocyte chemoattractant protein 1 levels were high. However, the exact nature of the role played by interferon-γ in regulating angiotensinogen and monocyte chemoattractant protein 1 expression has not been fully described. Therefore, the aim of this study was to investigate the role played by interferon-γ in angiotensinogen and monocyte chemoattractant protein 1 expression.
Methods: Primary cultured rat mesangial cells treated with 0-20 ng / mL interferon-γ for 2, 8 or 24 hours. angiotensinogen expression levels, monocyte chemoattractant protein 1, suppressor of cytokine signaling 1, suppressing the intracellular Janus kinase-signal transducer and activator of transcription signaling and activity of Janus kinase-signal transducer and activator of transcription pathway was evaluated by reverse transcriptase polymerase chain reaction and Western blot analysis.
Results: Interferon-γ increase the expression of angiotensinogen in mesangial cells was observed following the augmentation maximum 5 ng / mL interferon-γ at 8 hours of treatment (1.87 ± 0.05, mRNA, relative ratio). further increases reduced or no use of higher concentrations of interferon-γ. Following treatment, monocyte chemoattractant protein 1 expression is induced by a linear dose-dependent manner (6.85 ± 0.62-fold at 20 ng / mL interferon-γ at 24 hours). In addition, interferon-γ induced STAT1 phosphorylation and suppressor of cytokine signaling 1 expression with a linear dose-dependent manner.
Oppression STAT1 and suppressor of cytokine signaling 1 expression by small interference RNA angiotensinogen facilitated increased expression of interferon-γ-induced, suggesting that these two factors negatively regulate the expression of angiotensinogen. Conversely, an increase in interferon-γ-induced monocyte chemoattractant protein 1 expression was attenuated in STAT1-deficient mesangial cells, suggesting that STAT1 positively regulates monocyte chemoattractant protein 1 expression in mesangial cells.
Conclusion: These results indicate that while interferon-γ improve both angiotensinogen and monocyte chemoattractant protein 1 expression, STAT1 opponent played a role in the regulation of each of the factors in mesangial cells.
STAT1 regulates interferon-γ-induced angiotensinogen and MCP-1 expression in a bidirectional manner in primary cultured mesangial cells
Interferon and virus induces novel primate-specific isoform dACE2 and not SARS-CoV-2 receptor ACE2
acute respiratory syndrome-2 coronavirus (SARS-CoV-2), which causes COVID-19, take advantage of the angiotensin-converting enzyme 2 (ACE2) to enter the target cells. ACE2 has been proposed as interferon-stimulated genes (ISG). Thus, the interferon-induced variability in the level of ACE2 expression may be important for susceptibility to COVID-19 or its results. Here, we report the discovery of a new isoform, primate-specific ACE2, which we refer to as deltaACE2 (dACE2).
We show that dACE2, but not ACE2, the ISG. In vitro, dACE2, which has 356 amino acid N-terminal, is non-functional in binding of SARS-CoV spike protein-2 and as Karboksipeptidase A. Our results reconcile the current knowledge on the expression of ACE2 and suggested that the ISG-type induction dACE2 IFN-high in the conditions created by the treatment, the inflammatory tumor microenvironment, or virus co-infection is unlikely to affect the cellular influx of SARS-CoV-2 and promote infection.
Description: Recombinant Interferon gamma is a disulfide-linked monomer protein consisting of 144 amino acid residues, and migrates as an approximately 17 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human Interferon-gamma mature chain was expressed in E. coli.
Description: Recombinant Interferon gamma is a disulfide-linked monomer protein consisting of 144 amino acid residues, and migrates as an approximately 17 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human Interferon-gamma mature chain was expressed in E. coli.
Description: A competitive ELISA for quantitative measurement of Human Interferon β in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Interferon β in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Interferon β in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: Recombinant Interferon alpha 2a is a disulfide-linked monomeric protein consisting of 166 amino acid residues, and migrates as an approximately 19 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human Interferon-alpha mature chain was expressed in E. coli.
Description: Recombinant Interferon alpha 2a is a disulfide-linked monomeric protein consisting of 166 amino acid residues, and migrates as an approximately 19 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human Interferon-alpha mature chain was expressed in E. coli.
Description: Recombinant Interferon-alpha 2b is a disulfide-linked monomeric protein consisting of 166 amino acid residues. Interefron alpha 2b migrates as an approximately 19 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human Interferon-alpha mature chain was expressed in E. coli.
Description: Recombinant Interferon-alpha 2b is a disulfide-linked monomeric protein consisting of 166 amino acid residues. Interefron alpha 2b migrates as an approximately 19 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human Interferon-alpha mature chain was expressed in E. coli.
Description: Ubiquitin-Like Protein ISG15 (ISG15) is a ubiquitin-like protein that becomes conjugated to many cellular proteins upon activation by interferon-alpha and -beta. Several functions have been ascribed to the encoded protein, including chemotactic activity towards neutrophils, direction of ligated target proteins to intermediate filaments, cell-to-cell signaling, and antiviral activity during viral infections. While conjugates of this protein have been found to be noncovalently attached to intermediate filaments, this protein is sometimes secreted. ISG15 becomes conjugated to a diverse set of proteins after IFN-alpha/beta stimulation or microbial challenge. The functions or biochemical consequences ISG15 conjugation to proteins are not yet known, but it appears that this modification does not target proteins for proteasomal degradation. ISG15 shows specific chemotactic activity towards neutrophils and activates them to induce release of eosinophil chemotactic factors. Upon interferon treatment, ISG15 can be detected in both free and conjugated forms, and is secreted from monocytes and lymphocytes where it can function as a cytokine. In the cell, ISG15 co-localizes with intermediate filaments and ISGylation may modulate the JAK-STAT pathway or certain aspects of neurological disease.
Description: A competitive ELISA for quantitative measurement of Human Interferon β1b in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Interferon β1b in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Interferon β1b in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Interferon β1a in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Interferon β1a in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Interferon β1a in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Human Interferon ω1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Human Interferon ω1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Human Interferon ω1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Human Interferon alpha in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Human Interferon alpha in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Human Interferon alpha in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: Quantitativesandwich ELISA kit for measuring Human Interferon γ , IFN-γ in samples from serum, cell culture supernates, urine, tissue homogenates, cerebrospinalfluid (CSF). A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativesandwich ELISA kit for measuring Human Interferon γ , IFN-γ in samples from serum, cell culture supernates, urine, tissue homogenates, cerebrospinalfluid(CSF). Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: Quantitativesandwich ELISA kit for measuring Human Interferon α, IFN-α in samples from serum, plasma, cell culture supernates, urine, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativesandwich ELISA kit for measuring Human Interferon α, IFN-α in samples from serum, plasma, cell culture supernates, urine, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: A competitive ELISA for quantitative measurement of Human Interferon Alpha 21 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Interferon Alpha 21 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Interferon Alpha 21 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: Enzyme-linked immunosorbent assay kit for quantification of Human Interferon beta in samples from serum, plasma, tissue homogenates and other biological fluids.
Description: Interferon-gamma(IFN-gamma) is an inflammatory cytokine that has been implicated in the development of fibrosis in inflamed tissues. The production of IFN-gamma, which is under genetic control, can influence the development of fibrosis in lung allografts. IFN-gamma is also produced by natural killer(NK) cells and most prominently by CD8 cytotoxic T cells, and is vital for the control of microbial pathogens. Interferon gamma is believed to be crucial for host defence against many infections. Genetically determined variability in IFN-gamma and expression might be important for the development of tuberculosis. IFN-gamma activates human macrophage oxidative metabolism and antimicrobial activity. In addition to having antiviral activity, IFN-gamma has important immunoregulatory functions. IFN-gamma plays an important role in the control of neointima proliferation.
Description: A sandwich quantitative ELISA assay kit for detection of Human Interferon Beta (IFNb) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Human Interferon Beta (IFNb) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Interferon Gamma (IFNg) in serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Interferon Gamma (IFNg) in serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Interferon Gamma (IFNg) in serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Interferon Gamma (IFNg) in serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
Description: Double-antibody Sandwich chemiluminescent immunoassay for detection of Human Interferon Gamma (IFNg)Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Interferon Beta (IFNb) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Interferon Beta (IFNb) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Interferon Beta (IFNb) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Interferon Beta (IFNb) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Interferon Beta (IFNb) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Interferon Alpha (IFNa) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Interferon Alpha (IFNa) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Interferon Alpha (IFNa) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Interferon Alpha (IFNa) in serum, plasma and other biological fluids.
Description: Double-antibody Sandwich chemiluminescent immunoassay for detection of Human Interferon Alpha (IFNa)Serum, plasma and other biological fluids
Description: A competitive ELISA for quantitative measurement of Human Interferon γ （IFNγ ） in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Interferon γ （IFNγ ） in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Interferon γ （IFNγ ） in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: Human IFN-γ ELISA development kit contains the key components required for the quantitative measurement of natural and/or recombinant IFN-γ in a sandwich ELISA format within the range of 80-1,600 pg/mL. Using the ELISA protocol described below, this kit provides sufficient reagents to assay IFN-γ in approximately 1,500 ELISA plate wells.
Description: This assay is a sandwich Enzyme Linked-Immuno-Sorbent Assay(ELISA). It is developed for quantitative ; measurement of Human IFNG in serum, plasma and other biological fluids.
Description: Quantitativesandwich ELISA kit for measuring Human Interferon kappa (IFNK) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativesandwich ELISA kit for measuring Human Interferon kappa(IFNK) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: A sandwich quantitative ELISA assay kit for detection of Human Interferon Alpha (IFNa) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Human Interferon Alpha (IFNa) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Human Interferon Gamma (IFNg) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Human Interferon Gamma (IFNg) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich ELISA for quantitative measurement of Human Interferon lambda-3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Human Interferon lambda-3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Human Interferon lambda-3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Interferon Alpha (IFNa) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Interferon Alpha (IFNa) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Interferon Alpha (IFNa) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Interferon Alpha (IFNa) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Interferon Alpha (IFNa) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Interferon Gamma (IFNg) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Interferon Gamma (IFNg) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Interferon Gamma (IFNg) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Interferon Gamma (IFNg) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Interferon Gamma (IFNg) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.
Description: Quantitativesandwich ELISA kit for measuring Human Interferon β, IFN-β/IFNB in samples from serum, urine, cell culture supernates, tissue homogenates, cerebrospinalfluid (CSF). A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativesandwich ELISA kit for measuring Human Interferon β, IFN-β/IFNB in samples from serum, urine, cell culture supernates, tissue homogenates, cerebrospinalfluid(CSF). Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Development and research into new therapies that selectively and effectively destroy tumor cells that overexpress ERBB2 receptor is a pressing task. More recently, research into the use of type I interferon in the treatment of cancer has been intensified.
Ricardo
