Objective: Intrarenal interferon-γ contributed significantly to the development of glomerular injury in which angiotensinogen and monocyte chemoattractant protein 1 levels were high. However, the exact nature of the role played by interferon-γ in regulating angiotensinogen and monocyte chemoattractant protein 1 expression has not been fully described. Therefore, the aim of this study was to investigate the role played by interferon-γ in angiotensinogen and monocyte chemoattractant protein 1 expression.
Methods: Primary cultured rat mesangial cells treated with 0-20 ng / mL interferon-γ for 2, 8 or 24 hours. angiotensinogen expression levels, monocyte chemoattractant protein 1, suppressor of cytokine signaling 1, suppressing the intracellular Janus kinase-signal transducer and activator of transcription signaling and activity of Janus kinase-signal transducer and activator of transcription pathway was evaluated by reverse transcriptase polymerase chain reaction and Western blot analysis.
Results: Interferon-γ increase the expression of angiotensinogen in mesangial cells was observed following the augmentation maximum 5 ng / mL interferon-γ at 8 hours of treatment (1.87 ± 0.05, mRNA, relative ratio). further increases reduced or no use of higher concentrations of interferon-γ. Following treatment, monocyte chemoattractant protein 1 expression is induced by a linear dose-dependent manner (6.85 ± 0.62-fold at 20 ng / mL interferon-γ at 24 hours). In addition, interferon-γ induced STAT1 phosphorylation and suppressor of cytokine signaling 1 expression with a linear dose-dependent manner.
Oppression STAT1 and suppressor of cytokine signaling 1 expression by small interference RNA angiotensinogen facilitated increased expression of interferon-γ-induced, suggesting that these two factors negatively regulate the expression of angiotensinogen. Conversely, an increase in interferon-γ-induced monocyte chemoattractant protein 1 expression was attenuated in STAT1-deficient mesangial cells, suggesting that STAT1 positively regulates monocyte chemoattractant protein 1 expression in mesangial cells.
Conclusion: These results indicate that while interferon-γ improve both angiotensinogen and monocyte chemoattractant protein 1 expression, STAT1 opponent played a role in the regulation of each of the factors in mesangial cells.
STAT1 regulates interferon-γ-induced angiotensinogen and MCP-1 expression in a bidirectional manner in primary cultured mesangial cells
Interferon and virus induces novel primate-specific isoform dACE2 and not SARS-CoV-2 receptor ACE2
acute respiratory syndrome-2 coronavirus (SARS-CoV-2), which causes COVID-19, take advantage of the angiotensin-converting enzyme 2 (ACE2) to enter the target cells. ACE2 has been proposed as interferon-stimulated genes (ISG). Thus, the interferon-induced variability in the level of ACE2 expression may be important for susceptibility to COVID-19 or its results. Here, we report the discovery of a new isoform, primate-specific ACE2, which we refer to as deltaACE2 (dACE2).
We show that dACE2, but not ACE2, the ISG. In vitro, dACE2, which has 356 amino acid N-terminal, is non-functional in binding of SARS-CoV spike protein-2 and as Karboksipeptidase A. Our results reconcile the current knowledge on the expression of ACE2 and suggested that the ISG-type induction dACE2 IFN-high in the conditions created by the treatment, the inflammatory tumor microenvironment, or virus co-infection is unlikely to affect the cellular influx of SARS-CoV-2 and promote infection.
Description: Recombinant Interferon gamma is a disulfide-linked monomer protein consisting of 144 amino acid residues, and migrates as an approximately 17 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human Interferon-gamma mature chain was expressed in E. coli.
Description: Recombinant Interferon gamma is a disulfide-linked monomer protein consisting of 144 amino acid residues, and migrates as an approximately 17 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human Interferon-gamma mature chain was expressed in E. coli.
Description: Recombinant Interferon alpha 2a is a disulfide-linked monomeric protein consisting of 166 amino acid residues, and migrates as an approximately 19 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human Interferon-alpha mature chain was expressed in E. coli.
Description: Recombinant Interferon alpha 2a is a disulfide-linked monomeric protein consisting of 166 amino acid residues, and migrates as an approximately 19 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human Interferon-alpha mature chain was expressed in E. coli.
Description: Recombinant Interferon-alpha 2b is a disulfide-linked monomeric protein consisting of 166 amino acid residues. Interefron alpha 2b migrates as an approximately 19 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human Interferon-alpha mature chain was expressed in E. coli.
Description: Recombinant Interferon-alpha 2b is a disulfide-linked monomeric protein consisting of 166 amino acid residues. Interefron alpha 2b migrates as an approximately 19 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human Interferon-alpha mature chain was expressed in E. coli.
Description: ClinMax™ ELISA Kit is convenient ready-to-use immunoassay Kit, specifically designed to quantitate human IFN-gamma that is present in complex biological samples, such as human serum, plasma, and cell culture supernates. A comprehensive validation of the ELISA method was performed following the ICH M10 on bioanalytical method validation and the FDA’s bioanalytical method validation guidance for industry. This validation included assessments of linearity, accuracy, precision, dilution linearity, recovery, and the hook effect. For details information, please refer to the DS. ClinMax™ ELISA Kits are manufactured in a GMP-certified facility and comply to the ISO 13485 standard, ensuring a high level of quality and reliability.
Description: A competitive ELISA for quantitative measurement of Human Interferon β in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Interferon β in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Interferon β in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Interferon β1b in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Interferon β1b in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Interferon β1b in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Interferon β1a in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Interferon β1a in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Interferon β1a in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Human Interferon ω1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Human Interferon ω1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Human Interferon ω1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Human Interferon alpha in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Human Interferon alpha in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Human Interferon alpha in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.
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Development and research into new therapies that selectively and effectively destroy tumor cells that overexpress ERBB2 receptor is a pressing task. More recently, research into the use of type I interferon in the treatment of cancer has been intensified.
Ricardo
