Objective: Intrarenal interferon-γ contributed significantly to the development of glomerular injury in which angiotensinogen and monocyte chemoattractant protein 1 levels were high. However, the exact nature of the role played by interferon-γ in regulating angiotensinogen and monocyte chemoattractant protein 1 expression has not been fully described. Therefore, the aim of this study was to investigate the role played by interferon-γ in angiotensinogen and monocyte chemoattractant protein 1 expression.

Methods: Primary cultured rat mesangial cells treated with 0-20 ng / mL interferon-γ for 2, 8 or 24 hours. angiotensinogen expression levels, monocyte chemoattractant protein 1, suppressor of cytokine signaling 1, suppressing the intracellular Janus kinase-signal transducer and activator of transcription signaling and activity of Janus kinase-signal transducer and activator of transcription pathway was evaluated by reverse transcriptase polymerase chain reaction and Western blot analysis.

Results: Interferon-γ increase the expression of angiotensinogen in mesangial cells was observed following the augmentation maximum 5 ng / mL interferon-γ at 8 hours of treatment (1.87 ± 0.05, mRNA, relative ratio). further increases reduced or no use of higher concentrations of interferon-γ. Following treatment, monocyte chemoattractant protein 1 expression is induced by a linear dose-dependent manner (6.85 ± 0.62-fold at 20 ng / mL interferon-γ at 24 hours). In addition, interferon-γ induced STAT1 phosphorylation and suppressor of cytokine signaling 1 expression with a linear dose-dependent manner.

Oppression STAT1 and suppressor of cytokine signaling 1 expression by small interference RNA angiotensinogen facilitated increased expression of interferon-γ-induced, suggesting that these two factors negatively regulate the expression of angiotensinogen. Conversely, an increase in interferon-γ-induced monocyte chemoattractant protein 1 expression was attenuated in STAT1-deficient mesangial cells, suggesting that STAT1 positively regulates monocyte chemoattractant protein 1 expression in mesangial cells.

Conclusion: These results indicate that while interferon-γ improve both angiotensinogen and monocyte chemoattractant protein 1 expression, STAT1 opponent played a role in the regulation of each of the factors in mesangial cells.

Interferon and virus induces novel primate-specific isoform dACE2 and not SARS-CoV-2 receptor ACE2

acute respiratory syndrome-2 coronavirus (SARS-CoV-2), which causes COVID-19, take advantage of the angiotensin-converting enzyme 2 (ACE2) to enter the target cells. ACE2 has been proposed as interferon-stimulated genes (ISG). Thus, the interferon-induced variability in the level of ACE2 expression may be important for susceptibility to COVID-19 or its results. Here, we report the discovery of a new isoform, primate-specific ACE2, which we refer to as deltaACE2 (dACE2).

We show that dACE2, but not ACE2, the ISG. In vitro, dACE2, which has 356 amino acid N-terminal, is non-functional in binding of SARS-CoV spike protein-2 and as Karboksipeptidase A. Our results reconcile the current knowledge on the expression of ACE2 and suggested that the ISG-type induction dACE2 IFN-high in the conditions created by the treatment, the inflammatory tumor microenvironment, or virus co-infection is unlikely to affect the cellular influx of SARS-CoV-2 and promote infection.

Human CellExp? Thrombomodulin, Human Recombinant
7215-10	Biovision		EUR 240

Human CellExp? Thrombomodulin, Human Recombinant
7215-50	Biovision		EUR 860

Human CellExp? BAFF, human recombinant
7222-10	Biovision		EUR 256

Human CellExp? SHH, human recombinant
7223-10	Biovision		EUR 213

Human CellExp? Olfactomedin, human recombinant
7225-10	Biovision		EUR 278

Human CellExp? EGF, human recombinant
7228-10	Biovision		EUR 294

Human CellExp? ADAM12, human recombinant
7239-10	Biovision		EUR 256

Human CellExp? DKK3, human recombinant
7243-50	Biovision		EUR 343

Human CellExp? SPAM1, human recombinant
7247-10	Biovision		EUR 392

Human CellExp? SPAM1, human recombinant
7247-50	Biovision		EUR 1164

Human CellExp? UPA, human recombinant
7248-10	Biovision		EUR 332

Human CellExp? UPA, human recombinant
7248-50	Biovision		EUR 1104

Human CellExp? Furin, human recombinant
7249-10	Biovision		EUR 457

Human CellExp? GAD1, human recombinant
7250-10	Biovision		EUR 316

Human CellExp? PCSK9, human recombinant
7265-20	Biovision		EUR 408

Human CellExp? LIF, human recombinant
7267-10	Biovision		EUR 387

Human CellExp? CD36, human recombinant
7371-10	Biovision		EUR 245

Human CellExp? CD36, human recombinant
7371-50	Biovision		EUR 697

Human CellExp? CD80, human recombinant
7383-10	Biovision		EUR 234

Human CellExp? CD80, human recombinant
7383-50	Biovision		EUR 675

Human CellExp? CD47, human recombinant
7385-10	Biovision		EUR 278

Human CellExp? TNFRII, human recombinant
7394-10	Biovision		EUR 256

Human CellExp? SERPIND1, human recombinant
7411-10	Biovision		EUR 289

Human CellExp? VTCN1, human recombinant
7412-10	Biovision		EUR 245

Human CellExp? VTCN1, human recombinant
7412-50	Biovision		EUR 697

Human CellExp? CD23, human recombinant
7435-10	Biovision		EUR 262

Human CellExp? CD23, human recombinant
7435-50	Biovision		EUR 718

Human CellExp? TIMP1, human recombinant
7440-10	Biovision		EUR 305

Human CellExp? EPHB4, human recombinant
7451-100	Biovision		EUR 718

Human CellExp? EPHB4, human recombinant
7451-20	Biovision		EUR 272

Human CellExp? TFPI, human recombinant
7453-10	Biovision		EUR 283

Human CellExp? CD147, human recombinant
7455-10	Biovision		EUR 245

Human CellExp? CD147, human recombinant
7455-50	Biovision		EUR 697

Human CellExp? FOLR1, human recombinant
7456-10	Biovision		EUR 256

Human CellExp? FOLR1, human recombinant
7456-50	Biovision		EUR 729

Human CellExp? CD155, human recombinant
7462-10	Biovision		EUR 245

Human CellExp? CD155, human recombinant
7462-50	Biovision		EUR 697

Human CellExp? VLDLR, human recombinant
7464-10	Biovision		EUR 245

Human CellExp? VLDLR, human recombinant
7464-50	Biovision		EUR 697

Human CellExp? CADM1, human recombinant
7465-10	Biovision		EUR 245

Human CellExp? CADM1, human recombinant
7465-50	Biovision		EUR 697

Human CellExp? Transferrin, human recombinant
7467-100	Biovision		EUR 751

Human CellExp? Transferrin, human recombinant
7467-20	Biovision		EUR 278

Human CellExp? FOLR2, human recombinant
7471-10	Biovision		EUR 234

Human CellExp? FOLR2, human recombinant
7471-50	Biovision		EUR 669

Human CellExp? LDLR, human recombinant
7472-10	Biovision		EUR 300

Human CellExp? LDLR, human recombinant
7472-50	Biovision		EUR 827

Human CellExp? TFPI2, human recombinant
7481-10	Biovision		EUR 300

Human CellExp? RBP4, human recombinant
7563-10	Biovision		EUR 311

Human CellExp? RBP4, human recombinant
7563-50	Biovision		EUR 865

Human CellExp? UNC5B, human recombinant
7576-10	Biovision		EUR 272

Human CellExp?CD4, human recombinant
7834-100	Biovision		EUR 718

Human CellExp?CD4, human recombinant
7834-20	Biovision		EUR 267

Human CellExp? TIGIT, human recombinant
8015-10	Biovision		EUR 349

Human CellExp? Azurocidin, human recombinant
8017-20	Biovision		EUR 267

Human CellExp? CD163, Human recombinant
9226-10	Biovision		EUR 305

Human CellExp? CD163, Human recombinant
9226-50	Biovision		EUR 773

Human CellExp? BTLA, Human recombinant
9228-10	Biovision		EUR 289

Human CellExp? BTLA, Human recombinant
9228-50	Biovision		EUR 735

Human CellExp? CD28, Human recombinant
9237-10	Biovision		EUR 224

Human CellExp? CD28, Human recombinant
9237-100	Biovision		EUR 724

Human CellExp? CD19, Human recombinant
9246-10	Biovision		EUR 267

Human CellExp? CD19, Human recombinant
9246-25	Biovision		EUR 457

Human CellExp? Progranulin, Human Recombinant
4738-10	Biovision		EUR 305

Human CellExp? Progranulin, Human Recombinant
4738-100	Biovision		EUR 2273

Human CellExp? Renin, Human Recombinant
6300-10	Biovision		EUR 164

Human CellExp? Renin, Human Recombinant
6300-100	Biovision		EUR 784

Human CellExp? Renin, Human Recombinant
6300-50	Biovision		EUR 501

Human CellExp? EPO, Human Recombinant
6447-10	Biovision		EUR 539

Human CellExp? EPO, Human Recombinant
6447-50	Biovision		EUR 1681

Human CellExp? HGF, Human Recombinant
6456-10	Biovision		EUR 387

Human CellExp? HGF, Human Recombinant
6456-50	Biovision		EUR 1398

Human CellExp? HGH, Human Recombinant
6457-10	Biovision		EUR 338

Human CellExp? HGH, Human Recombinant
6457-50	Biovision		EUR 1213

Human CellExp? Noggin, Human Recombinant
6474-10	Biovision		EUR 300

Human CellExp? Noggin, Human Recombinant
6474-50	Biovision		EUR 1072

Human CellExp? SCF, Human Recombinant
6478-10	Biovision		EUR 343

Human CellExp? SCF, Human Recombinant
6478-50	Biovision		EUR 1333

Human CellExp? TPO, Human Recombinant
6483-10	Biovision		EUR 365

Human CellExp? TPO, Human Recombinant
6483-50	Biovision		EUR 1980

Human CellExp? VAPB, Human recombinant
P1135-10	Biovision		EUR 131

Human CellExp? VAPB, Human recombinant
P1135-50	Biovision		EUR 370

Human CellExp? Transthyretin, Human recombinant
P1138-20	Biovision		EUR 185

Human CellExp? Transthyretin, Human recombinant
P1138-50	Biovision		EUR 392

Human CellExp? Sclerostin, human recombinant
P1145-10	Biovision		EUR 153

Human CellExp? Sclerostin, human recombinant
P1145-50	Biovision		EUR 533

Human CellExp? SLITRK6, human recombinant
P1146-10	Biovision		EUR 142

Human CellExp? SLITRK6, human recombinant
P1146-50	Biovision		EUR 446

Human CellExp? LILRB2, human recombinant
P1147-10	Biovision		EUR 131

Human CellExp? LILRB2, human recombinant
P1147-50	Biovision		EUR 403

Human CellExp? ROR1, human recombinant
P1152-10	Biovision		EUR 142

Human CellExp? ROR1, human recombinant
P1152-50	Biovision		EUR 446

Human CellExp? DLL3, Human Recombinant
P1165-10	Biovision		EUR 566

Human CellExp? DLK1, Human Recombinant
P1166-10	Biovision		EUR 555

Human CellExp? PTPRD, Human Recombinant
P1184-10	Biovision		EUR 153

Human CellExp? PTPRD, Human Recombinant
P1184-50	Biovision		EUR 512

Human CellExp? KRAS, Human Recombinant
P1198-10	Biovision		EUR 142

Human CellExp? KRAS, Human Recombinant
P1198-1000	Biovision		EUR 2654

Human CellExp? KRAS, Human Recombinant
P1198-250	Biovision		EUR 892

Human CellExp? KRAS, Human Recombinant
P1198-50	Biovision		EUR 262

Human CellExp? Hemopexin, Human Recombinant
P1210-10	Biovision		EUR 196

Human CellExp? Hemopexin, Human Recombinant
P1210-50	Biovision		EUR 664

Human CellExp? ECE1, Human Recombinant
P1227-10	Biovision		EUR 251

Human CellExp? ECE1, Human Recombinant
P1227-50	Biovision		EUR 1012

Human CellExp? CD8b, Human Recombinant
P1244-10	Biovision		EUR 240

Human CellExp? CD8b, Human Recombinant
P1244-1000	Biovision		Ask for price

Human CellExp? CD8b, Human Recombinant
P1244-250	Biovision		Ask for price

Human CellExp? CD8b, Human Recombinant
P1244-50	Biovision		EUR 805

Human CellExp? ROR2, Human Recombinant
P1254-10	Biovision		EUR 229

Human CellExp? VEGF110, Human Recombinant
P1258-10	Biovision		EUR 479

Human CellExp? Prolactin, Human Recombinant
P1311-10	Biovision		EUR 153

Human CellExp? Prolactin, Human Recombinant
P1311-50	Biovision		EUR 240

Human CellExp? Dermatopontin, Human Recombinant
P1319-10	Biovision		EUR 338

Human CellExp? CD177, Human Recombinant
P1410-10	Biovision		EUR 153

Human CellExp? CD177, Human Recombinant
P1410-50	Biovision		EUR 392

Human CellExp? PM20D1, Human Recombinant
P1430-10	Biovision		EUR 403

Human CellExp? DLL3, Human Recombinant
P1463-10	Biovision		EUR 169

Human CellExp? DLL3, Human Recombinant
P1463-50	Biovision		EUR 501

Human CellExp? Properdin, Human Recombinant
P1470-10	Biovision		EUR 164

Human CellExp? Properdin, Human Recombinant
P1470-50	Biovision		EUR 479

Human CellExp? CD38, human recombinant
P1014-10	Biovision		EUR 207

Human CellExp? CD38, human recombinant
P1014-50	Biovision		EUR 479

Human CellExp? CD45, human recombinant
P1015-10	Biovision		EUR 251

Human CellExp? CD45, human recombinant
P1015-25	Biovision		EUR 457

Human CellExp? VSIG4, human recombinant
P1018-10	Biovision		EUR 207

Human CellExp? VSIG4, human recombinant
P1018-50	Biovision		EUR 479

Human CellExp? TrkA, human recombinant
P1020-10	Biovision		EUR 175

Human CellExp? NOV, human recombinant
P1022-10	Biovision		EUR 196

Human CellExp? NOV, human recombinant
P1022-25	Biovision		EUR 294

Human CellExp? EGFRvIII, human recombinant
P1082-10	Biovision		EUR 283

Human CellExp? LOXL2, human recombinant
P1084-10	Biovision		EUR 153

Human CellExp? LOXL2, human recombinant
P1084-50	Biovision		EUR 533

Human CellExp? CD83, Human recombinant
P1109-10	Biovision		EUR 131

Human CellExp? CD83, Human recombinant
P1109-50	Biovision		EUR 403

Human CellExp? CD160, human recombinant
P1110-10	Biovision		EUR 142

Human CellExp? CD160, human recombinant
P1110-50	Biovision		EUR 392

Human CellExp? CNDP1, Human recombinant
P1125-10	Biovision		EUR 207

Human CellExp? CNDP1, Human recombinant
P1125-50	Biovision		EUR 838

Human-to-Human Blocking Reagent
HTH008	ScyTek Laboratories	8 ml	EUR 76

Human-to-Human Blocking Reagent
HTH015	ScyTek Laboratories	15 ml	EUR 83

Human-to-Human Blocking Reagent
HTH100	ScyTek Laboratories	100 ml	EUR 245

Human CellExp? IL-34, Human Recombinant
7108-10	Biovision		EUR 278

Human CellExp? IL-34, Human Recombinant
7108-50	Biovision		EUR 1132

Human CellExp? DKK-1, Human Recombinant
7132-10	Biovision		EUR 278

Human CellExp? DKK-1, Human Recombinant
7132-50	Biovision		EUR 1132

Human CellExp? sDLL-1, Human Recombinant
7133-10	Biovision		EUR 207

Human CellExp? sDLL-1, Human Recombinant
7133-50	Biovision		EUR 675

Human CellExp? sDLL-4, Human Recombinant
7134-10	Biovision		EUR 207

Human CellExp? sDLL-4, Human Recombinant
7134-50	Biovision		EUR 675

Human CellExp? PECAM-1, Human Recombinant
7184-10	Biovision		EUR 153

Human CellExp? PECAM-1, Human Recombinant
7184-50	Biovision		EUR 381

Human CellExp? VCAM-1, Human Recombinant
7211-10	Biovision		EUR 142

Human CellExp? VCAM-1, Human Recombinant
7211-50	Biovision		EUR 381

Human CellExp? TLR-3, Human Recombinant
7220-10	Biovision		EUR 207

Human CellExp? TLR-3, Human Recombinant
7220-50	Biovision		EUR 675

Human CellExp? IL-17E, human recombinant
7224-10	Biovision		EUR 370

Human CellExp? VEGF-B, human recombinant
7230-10	Biovision		EUR 370

Human CellExp? VEGF-C, human recombinant
7231-10	Biovision		EUR 370

Human CellExp? Granzyme B, human recombinant
7233-10	Biovision		EUR 555

Human CellExp? SERPINA1 /A1AT, human recombinant
7235-10	Biovision		EUR 305

Human CellExp? SERPIN A8, human recombinant
7236-10	Biovision		EUR 316

Human CellExp? HMGB1 /HMG1, human recombinant
7240-10	Biovision		EUR 229

Human CellExp? HMGB1 /HMG1, human recombinant
7240-50	Biovision		EUR 582

Human CellExp? MMP-1, human recombinant
7244-10	Biovision		EUR 316

Human CellExp? MMP-1, human recombinant
7244-50	Biovision		EUR 1050

Human CellExp? MMP-2, human recombinant
7245-10	Biovision		EUR 332

Human CellExp? MMP-2, human recombinant
7245-50	Biovision		EUR 1104

Human CellExp? MMP-9, human recombinant
7246-10	Biovision		EUR 343

Human CellExp? MMP-9, human recombinant
7246-1000	Biovision		Ask for price

Human CellExp? MMP-9, human recombinant
7246-250	Biovision		EUR 3878

Human CellExp? MMP-9, human recombinant
7246-50	Biovision		EUR 1137

Human CellExp? IFN-gamma, human recombinant
7271-10	Biovision		EUR 256

Human CellExp? IL-22, human recombinant
7272-10	Biovision		EUR 359

Human CellExp? IL-15, human recombinant
7273-10	Biovision		EUR 512

Human CellExp? IL-7, human recombinant
7274-10	Biovision		EUR 490

Human CellExp? PDGF-BB, human recombinant
7276-10	Biovision		EUR 419

Human CellExp? Cathepsin S, human recombinant
7277-10	Biovision		EUR 349

Human CellExp? Cathepsin S, human recombinant
7277-50	Biovision		EUR 1104

Human CellExp?LAG3 /CD223, human recombinant
7278-10	Biovision		EUR 267

Human CellExp?LAG3 /CD223, human recombinant
7278-50	Biovision		EUR 718

Human CellExp? CD360 / IL21R, human recombinant
7373-100	Biovision		EUR 697

Human CellExp? CD360 / IL21R, human recombinant
7373-20	Biovision		EUR 245

Human CellExp? CD306 / LAIR2, human recombinant
7377-10	Biovision		EUR 245

Human CellExp? CD306 / LAIR2, human recombinant
7377-50	Biovision		EUR 697

Human CellExp? CD84 / SLAMF5, human recombinant
7378-10	Biovision		EUR 245

Human CellExp? CD84 / SLAMF5, human recombinant
7378-50	Biovision		EUR 697

Human CellExp? LAMP1/CD107a, human recombinant
7381-10	Biovision		EUR 245

Human CellExp? LAMP1/CD107a, human recombinant
7381-50	Biovision		EUR 697

Human CellExp? CD74/DHLAG, human recombinant
7387-10	Biovision		EUR 278

Human CellExp? CD74/DHLAG, human recombinant
7387-50	Biovision		EUR 751

Development and research into new therapies that selectively and effectively destroy tumor cells that overexpress ERBB2 receptor is a pressing task. More recently, research into the use of type I interferon in the treatment of cancer has been intensified.